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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1977-10-28
pubmed:abstractText
Aster formation was studied in sea-urchin eggs artificially activated by Loeb's double method. The number of asters found in an activated egg ranged from 15-40. Observation by electron microscopy revealed the presence of a centriole in a high percentage of isolated asters, strongly suggesting that these centrioles are formed de novo in the activated eggs. Using negatively stained specimens, which were isolated with 5% hexylene glycol solution containing buffer and EGTA, the whole aster could be examined by electron microscopy. A microtubular framework in the aster, extending radially from the astral centre was observed. The effect of mitotic inhibitors on aster formation was found to be similar to that on cell division.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9533
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
203-16
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1977
pubmed:articleTitle
Studies on the de novo formation of centrioles: aster formation in the activated eggs of sea urchin.
pubmed:publicationType
Journal Article