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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1997-1-24
|
| pubmed:abstractText |
The recombinase activation genes, RAG-1 and RAG-2, are expressed together in immature T or B lymphocytes and possess activity to induce V(D)J rearrangement in T cell receptor (TCR) and Ig genes. In vertebrates, only Ig and TCR molecules are reported to have recombination in their development using multiple V, D, J component gene segments. Thus, expression of RAG genes are localized only in lymphoid organs and sites of extrathymic T cell differentiation. In this study, we have used RAG-1 and RAG-2 genes as markers of possible genetic recombination in developing murine preimplantation embryos, using the highly sensitive reverse transcriptase polymerase chain reaction (RT-PCR) technique and in situ hybridization. From 40 preimplantation embryos of various developmental stages we extracted RNA, reverse-transcribed it into cDNA and used it in RT-PCR studies. A PCR of 35 cycles disclosed expression of RAG-1 but not RAG-2 in morulae and blastocysts. Southern blot hybridization using a specific synthetic oligonucleotide probe for RAG-1 and RT-PCR with another primer pair identified RAG-1 expression in developing embryos. In situ hybridization using a cooled CCD camera also revealed localization of RAG-1 mRNA in blastocysts. We propose possible genetic recombination during late preimplantation murine embryogenesis which may contribute to the loss of totipotency and differentiation of inner cell mass and trophoectoderm.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0818-9641
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
74
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
52-6
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8934654-Animals,
pubmed-meshheading:8934654-Base Sequence,
pubmed-meshheading:8934654-Blotting, Southern,
pubmed-meshheading:8934654-DNA, Complementary,
pubmed-meshheading:8934654-Female,
pubmed-meshheading:8934654-Gene Expression,
pubmed-meshheading:8934654-Genes, RAG-1,
pubmed-meshheading:8934654-In Situ Hybridization,
pubmed-meshheading:8934654-Mice,
pubmed-meshheading:8934654-Mice, Inbred ICR,
pubmed-meshheading:8934654-Molecular Sequence Data,
pubmed-meshheading:8934654-Polymerase Chain Reaction
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Expression of the recombinase-activating gene (RAG-1) in murine early embryogenesis.
|
| pubmed:affiliation |
Department of Obstetrics and Gynaecology, Nihon University, Tokyo, Japan.
|
| pubmed:publicationType |
Journal Article
|