Linked Life Data

8934542

Source:http://linkedlifedata.com/resource/pubmed/id/8934542

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1997-1-7
pubmed:abstractText
Bruton's agammaglobulinemia tyrosine kinase (Btk) is a cytoplasmic tyrosine kinase involved in the human disease X-linked agammaglobulinemia (XLA). The gene is expressed in all hematopoietic cells with the exception of T-cells and plasma cells. For this expression pattern the first 280 bp upstream of the major transcriptional start site seems to be sufficient. In vitro footprinting analysis within this part of the promoter revealed two Sp1 binding sites as well as a PU-box. The transcription factor Spi-1/PU.1 as well as the closely related factor Spi-B bound to the PU-box in B-cells. In the erythroleukemia cell line K562, due to the absence of Spi-B, only PU.1 bound to the Btk promoter. Mutation of either site reduced the expression in transient transfection experiments. However, mutation of the PU box had no effect in the T-cell line Jurkat, where none of the Spi-1 family members is expressed. In addition Spi-B as well as PU.1 were able to transactivate Btk expression. In fetal liver of PU.1-/- mice, which lack lymphoid and myeloid cells, expression of Btk was reduced two- to threefold but not abolished. Collectively this study shows that expression of the Btk gene is regulated by the combined action of Sp1- and PU.1-family members.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:day
7
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1955-64
pubmed:dateRevised
2011-11-2
pubmed:meshHeading
pubmed-meshheading:8934542-Animals, pubmed-meshheading:8934542-B-Lymphocytes, pubmed-meshheading:8934542-Binding Sites, pubmed-meshheading:8934542-DNA-Binding Proteins, pubmed-meshheading:8934542-Gene Expression Regulation, pubmed-meshheading:8934542-Heterozygote, pubmed-meshheading:8934542-Humans, pubmed-meshheading:8934542-Jurkat Cells, pubmed-meshheading:8934542-Leukemia, Erythroblastic, Acute, pubmed-meshheading:8934542-Liver, pubmed-meshheading:8934542-Mice, pubmed-meshheading:8934542-Mice, Mutant Strains, pubmed-meshheading:8934542-Mutation, pubmed-meshheading:8934542-Promoter Regions, Genetic, pubmed-meshheading:8934542-Protein-Tyrosine Kinases, pubmed-meshheading:8934542-Proto-Oncogene Proteins, pubmed-meshheading:8934542-Sp1 Transcription Factor, pubmed-meshheading:8934542-Sp3 Transcription Factor, pubmed-meshheading:8934542-Trans-Activators, pubmed-meshheading:8934542-Transcription, Genetic, pubmed-meshheading:8934542-Transcription Factors, pubmed-meshheading:8934542-Transcriptional Activation, pubmed-meshheading:8934542-Tumor Cells, Cultured
pubmed:year
1996
pubmed:articleTitle
Cell specific expression of human Bruton's agammaglobulinemia tyrosine kinase gene (Btk) is regulated by Sp1- and Spi-1/PU.1-family members.
pubmed:affiliation
Center for BioTechnology, Karolinska Institute, Department of Bioscience at NOVUM, Huddinge, Sweden.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't