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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5292
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pubmed:dateCreated |
1996-12-26
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pubmed:databankReference |
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pubmed:abstractText |
The 3' ends of most eukaryotic messenger RNAs are generated by internal cleavage and polyadenylation. In mammals, there is a strict dependence of both reactions on the sequence AAUAAA, which occurs upstream of polyadenylation [poly(A)] sites and which is recognized by CPSF. In contrast, cis-acting signals for yeast 3'-end generation are highly divergent from those of mammals, suggesting that trans-acting factors other than poly(A) polymerase would not be conserved. The essential yeast protein Brr5/Ysh1 shows sequence similarity to subunits of mammalian CPSF and is required for 3'-end processing in vivo and in vitro. These results demonstrate a structural and functional conservation of the yeast and mammalian 3'-end processing machineries despite a lack of conservation of the cis sequences.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
29
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pubmed:volume |
274
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1511-4
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8929408-Amino Acid Sequence,
pubmed-meshheading:8929408-Animals,
pubmed-meshheading:8929408-Base Sequence,
pubmed-meshheading:8929408-Cattle,
pubmed-meshheading:8929408-Conserved Sequence,
pubmed-meshheading:8929408-Crystallography, X-Ray,
pubmed-meshheading:8929408-Fungal Proteins,
pubmed-meshheading:8929408-Molecular Sequence Data,
pubmed-meshheading:8929408-Mutation,
pubmed-meshheading:8929408-Poly A,
pubmed-meshheading:8929408-RNA, Fungal,
pubmed-meshheading:8929408-RNA, Messenger,
pubmed-meshheading:8929408-RNA Precursors,
pubmed-meshheading:8929408-RNA Processing, Post-Transcriptional,
pubmed-meshheading:8929408-RNA-Binding Proteins,
pubmed-meshheading:8929408-Saccharomyces cerevisiae,
pubmed-meshheading:8929408-mRNA Cleavage and Polyadenylation Factors
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pubmed:year |
1996
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pubmed:articleTitle |
Essential yeast protein with unexpected similarity to subunits of mammalian cleavage and polyadenylation specificity factor (CPSF).
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pubmed:affiliation |
Department of Biochemistry and Biophysics, UCSF School of Medicine, San Francisco, CA 94143-0448. guthrie@cgl.ucsf.edu.
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pubmed:publicationType |
Journal Article,
Comment,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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