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rdf:type |
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lifeskim:mentions |
umls-concept:C0002844,
umls-concept:C0007600,
umls-concept:C0040649,
umls-concept:C0041904,
umls-concept:C0086418,
umls-concept:C0162493,
umls-concept:C0332197,
umls-concept:C0600139,
umls-concept:C1367578,
umls-concept:C1508748,
umls-concept:C1518174,
umls-concept:C2677316
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pubmed:issue |
3
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|
pubmed:dateCreated |
1996-11-14
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|
pubmed:abstractText |
A theoretical pathway of transcriptional regulation of the androgen receptor (AR) gene is via a cAMP response element (CRE) present in its promoter region (-508 to -501). After 20 h of stimulation with 8-bromo-cAMP, AR mRNA was upregulated in LNCaP but not in either PC-3 or DU-145 cell lines. We have demonstrated that the level of CRE binding protein (CREB) was the same in all cell lines and that the putative AR-CRE forms specific and compatible protein interactions with CREB. The ability to regulate AR gene transcription via the second messenger pathway is lost in the PC-3 and DU-145 cell lines. This may be an important primary mechanism of androgen insensitivity in prostate cancer.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0014-5793
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
383
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
237-40
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8925904-Animals,
pubmed-meshheading:8925904-Base Sequence,
pubmed-meshheading:8925904-Cell Line,
pubmed-meshheading:8925904-Cell Nucleus,
pubmed-meshheading:8925904-Cyclic AMP Response Element-Binding Protein,
pubmed-meshheading:8925904-DNA Primers,
pubmed-meshheading:8925904-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:8925904-HeLa Cells,
pubmed-meshheading:8925904-Humans,
pubmed-meshheading:8925904-Male,
pubmed-meshheading:8925904-Molecular Sequence Data,
pubmed-meshheading:8925904-Polymerase Chain Reaction,
pubmed-meshheading:8925904-Prostatic Neoplasms,
pubmed-meshheading:8925904-Protein Biosynthesis,
pubmed-meshheading:8925904-RNA, Messenger,
pubmed-meshheading:8925904-Rabbits,
pubmed-meshheading:8925904-Receptors, Androgen,
pubmed-meshheading:8925904-Reticulocytes,
pubmed-meshheading:8925904-Second Messenger Systems,
pubmed-meshheading:8925904-Transcription, Genetic,
pubmed-meshheading:8925904-Tumor Cells, Cultured,
pubmed-meshheading:8925904-Up-Regulation
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pubmed:year |
1996
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pubmed:articleTitle |
Second messenger up-regulation of androgen receptor gene transcription is absent in androgen insensitive human prostatic carcinoma cell lines, PC-3 and DU-145.
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pubmed:affiliation |
Department of Histopathology, Royal Postgraduate Medical School Hammersmith Hospital, London, UK.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|
