Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
1996-11-8
|
pubmed:abstractText |
The production of 12-hydroxyeicosatetraenoic acid by the corneal epithelium of several species has been extensively reported yet the controversy over the exclusive production of the (S) epimer (a lipoxygenase-derived metabolite) endures. Incubation of calf corneal epithelial microsomes (3 mg/ml) with arachidonic acid and NADPH resulted in the formation of 12-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE). The synthesis of 12-HETE was inhibited by SKF-525A and clotrimazole, selective inhibitors of cytochrome P450 dependent activities, but not by indomethacin or BW-755C, inhibitors of cyclooxygenase and lipoxygenase activities, respectively. Chiral analysis revealed the presence of both enantiomers; however, the R isomer was the predominant one, i.e., 91 +/- 5% vs. 9 +/- 5% for the R and S enantiomers, respectively. Since the R enantiomer is the product of a cytochrome P450-mediated reaction, it suggests that the major metabolic activity in these microsomes is cytochrome P450-dependent and supports the claim for cytochrome P450 reactions in this ocular tissue.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:issn |
1080-7683
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
12
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
19-26
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:8925392-12-Hydroxy-5,8,10,14-eicosatetraenoic Acid,
pubmed-meshheading:8925392-Animals,
pubmed-meshheading:8925392-Arachidonic Acid,
pubmed-meshheading:8925392-Cattle,
pubmed-meshheading:8925392-Chromatography, High Pressure Liquid,
pubmed-meshheading:8925392-Cornea,
pubmed-meshheading:8925392-Cytochrome P-450 Enzyme System,
pubmed-meshheading:8925392-Endothelium, Corneal,
pubmed-meshheading:8925392-Microsomes,
pubmed-meshheading:8925392-NADP,
pubmed-meshheading:8925392-Stereoisomerism
|
pubmed:year |
1996
|
pubmed:articleTitle |
Chiral analysis of 12-hydroxyeicosatetraenoic acid formed by calf corneal epithelial microsomes.
|
pubmed:affiliation |
Department of Pharmacology, New York Medical College, Valhalla, USA.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|