Linked Life Data

8922948

Source:http://linkedlifedata.com/resource/pubmed/id/8922948

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1997-2-20
pubmed:abstractText
To elucidate the mechanism of ossification of the posterior longitudinal ligament (OPLL), we examined the serial changes in the intervertebral disc of tip-toe walking Yoshimura (twy) mouse. At the age of 6 weeks, the volume of the nucleus pulposus increased in all intervertebral discs causing anterior and posterior herniation. Secondary to this herniation, the cartilagineous tissue of the annulus fibrosus was disrupted and showed regenerative proliferation with PCNA-positive cartilagineous cells. These cells were S-100 positive and the matrix was positive for chondroitin-4-sulfate proteoglycan, indicating the development of calcification. At the age of 15 weeks, the regenerative cartilagineous tissue of the annulus fibrosus reached the posterior longitudinal ligament together with neovascularization and appearance of PCNA-positive proliferating primitive mesenchymal cells. These cells were considered to be osteoblasts since they were positive for alkaline phosphatase and the matrix contained type I collagen. Using electron microscopic X-ray analysis, vesicles present in the matrix of regenerative cartilagineous cells of the annulus fibrosus were confirmed to contain calcium phosphate crystals, also indicating the development of calcification. In conclusion, our serial analysis indicates that ossification of posterior longitudinal ligament in twy mouse was triggered by enlargement of the nucleus pulposus followed by herniation, disruption and regenerative proliferation of annulus fibrosus cartilagineous tissues. Enchondral ossification of the new annulus fibrosus cartilagineous cells and membraneous ossification by primitive osteoblasts proceed to the final ossification of the posterior longitudinal ligaments.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1121-760X
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
199-210
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:8922948-Alkaline Phosphatase, pubmed-meshheading:8922948-Animals, pubmed-meshheading:8922948-Calcification, Physiologic, pubmed-meshheading:8922948-Chondroitin Sulfate Proteoglycans, pubmed-meshheading:8922948-Collagen, pubmed-meshheading:8922948-Disease Models, Animal, pubmed-meshheading:8922948-Electron Probe Microanalysis, pubmed-meshheading:8922948-Immunohistochemistry, pubmed-meshheading:8922948-Intervertebral Disc, pubmed-meshheading:8922948-Mice, pubmed-meshheading:8922948-Mice, Inbred ICR, pubmed-meshheading:8922948-Microscopy, Electron, Scanning, pubmed-meshheading:8922948-Ossification of Posterior Longitudinal Ligament, pubmed-meshheading:8922948-Proliferating Cell Nuclear Antigen, pubmed-meshheading:8922948-S100 Proteins, pubmed-meshheading:8922948-Staining and Labeling, pubmed-meshheading:8922948-Tolonium Chloride
pubmed:year
1996
pubmed:articleTitle
Characteristics and mechanism of the ossification of posterior longitudinal ligament in the tip-toe walking Yoshimura (twy) mouse.
pubmed:affiliation
Department of Orthopaedic Surgery, Fukui Medical School, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't