Linked Life Data

8922406

Source:http://linkedlifedata.com/resource/pubmed/id/8922406

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
1997-1-6
pubmed:abstractText
The effects of dopamine (DA) on inhibitory transmission onto identified magnocellular neurons were examined in rat basal forebrain slices using whole-cell recording. IPSCs evoked by focal stimulation within basal forebrain nuclei were reversibly blocked by 10 microM bicuculline and had a decay time constant of 20.1 +/- 0.77 msec in the presence of 6-cyano-7-nitroquinoxalline-2,3-dione (5 mM). Bath application of DA reduced the amplitude of IPSCs up to 71.1 +/- 1.49% in a concentration-dependent manner between 0.003 and 1 mM (the IC50 value being 6.6 microM), without any effect on the holding current at -70 mV. DA (10 microM) reduced the frequency of miniature IPSCs (mIPSCs) recorded in the presence of TTX (0.5 microM), without affecting their mean amplitude, rise time, and decay time constant. Furthermore, the DA-induced effect on mIPSCs remained unaffected by 100 microM cadmium, suggesting a presynaptic mechanism independent of calcium influx. SKF 81297, a D1-like agonist, mimicked DA-induced effect on evoked IPSCs (IC50, 10.9 microM), whereas R(-)-TNPA or (-)-quinpirole, D2-like agonists (30 microM), had little or no effect on the amplitude of evoked IPSCs. R(+)-SCH 23390, a D1-like antagonist, antagonized the DA-induced effect on IPSCs (K(B) 0.82 microM), whereas S(-)-eticlopride, a D2-like antagonist, showed slight antagonism (K(B) 7.8 microM). Forskolin (10 microM) reduced the amplitude of evoked IPSCs to approximately 58% of the control and occluded the inhibitory effect of DA. These findings indicate that DA reduces inhibitory transmission onto magnocellular basal forebrain neurons by activating presynaptic D1-like receptors.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0270-6474
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7505-12
pubmed:dateRevised
2009-9-29
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Modulation of inhibitory transmission by dopamine in rat basal forebrain nuclei: activation of presynaptic D1-like dopaminergic receptors.
pubmed:affiliation
Department of Pharmacology, University College London, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't