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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1996-12-30
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pubmed:abstractText |
Type VIII collagen is a short-chain collagen with considerable similarity to type X collagen. We have generated chain-specific antibodies to the alpha 1 and alpha 2 chains of type VIII collagen, and used them as probes to examine the synthesis of type VIII collagen by vascular smooth muscle cells (VSMC). In addition, chain-specific oligonucleotides have been used in reverse transcriptase-PCR (RT-PCR) reactions with RNA extracted from cultured smooth muscle cells in culture and from freshly isolated vascular tissues. Radiolabelling of VSMC in culture and immunoprecipitation with chain-specific antibodies showed that both chains were expressed. Lower levels of type VIII collagen were found in adult VSMC than in neonatal VSMC. RT-PCR showed that both chains were expressed in tissues as well as cells in culture. The results indicate that type VIII collagen is a product of VSMC of normal adult vessels and is expressed at high levels by VSMC in vascular lesions.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-1702626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-1813143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-1831503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-1847345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-1860888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-1864364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-1869415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-1916105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-1999431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-2003978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-2019595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-2029894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-2104858,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-2226849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-2337296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-2376131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-2584206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-2649093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-3047147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-3081516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-3527259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-4850204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-567446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-6257289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-6275471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-6401740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-6630235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-6694361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-7574488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-7734329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8921010-8322888
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
319 ( Pt 3)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
993-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8921010-Adult,
pubmed-meshheading:8921010-Amino Acid Sequence,
pubmed-meshheading:8921010-Animals,
pubmed-meshheading:8921010-Aorta,
pubmed-meshheading:8921010-Base Sequence,
pubmed-meshheading:8921010-Cattle,
pubmed-meshheading:8921010-Cells, Cultured,
pubmed-meshheading:8921010-Collagen,
pubmed-meshheading:8921010-DNA Primers,
pubmed-meshheading:8921010-Fetus,
pubmed-meshheading:8921010-Humans,
pubmed-meshheading:8921010-Infant,
pubmed-meshheading:8921010-Intestinal Mucosa,
pubmed-meshheading:8921010-Muscle, Smooth, Vascular,
pubmed-meshheading:8921010-Oligonucleotides, Antisense,
pubmed-meshheading:8921010-Pepsin A,
pubmed-meshheading:8921010-Polymerase Chain Reaction,
pubmed-meshheading:8921010-Sudden Infant Death,
pubmed-meshheading:8921010-Swine
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pubmed:year |
1996
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pubmed:articleTitle |
Type VIII collagen is a product of vascular smooth-muscle cells in development and disease.
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pubmed:affiliation |
Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, School of Biological Sciences, U.K.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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