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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1996-12-30
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pubmed:abstractText |
Tumor necrosis factor alpha mediated cell death in L929 cells correlates with a late increase in reduction of the superoxide scavenger 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), suggesting an increase in MTT reduction per viable cell. This effect was studied in two TNF-sensitive and in five different TNF-resistant clones. Within 36 hrs TNF promoted a 7-fold increase in the reduction of MTT in TNF-sensitive cells. Exogenous ceramide induced a similar effect prior to cell death. Four of the five TNF-resistant clones were also resistant to ceramide and displayed no increase in MTT reduction with either TNF or ceramide. The remaining TNF-resistant clone was sensitive to ceramide, displaying an increase in MTT reduction. Our results suggest a late increase in superoxide production prior to cellular destruction during TNF and ceramide mediated cell death and support the notion that ceramide can serve as a second messenger for TNF in cell death.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
228
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
505-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8920943-Animals,
pubmed-meshheading:8920943-Cell Death,
pubmed-meshheading:8920943-Cell Survival,
pubmed-meshheading:8920943-Ceramides,
pubmed-meshheading:8920943-Clone Cells,
pubmed-meshheading:8920943-Coloring Agents,
pubmed-meshheading:8920943-Drug Resistance,
pubmed-meshheading:8920943-Free Radical Scavengers,
pubmed-meshheading:8920943-Kinetics,
pubmed-meshheading:8920943-L Cells (Cell Line),
pubmed-meshheading:8920943-Mice,
pubmed-meshheading:8920943-Mutagenesis,
pubmed-meshheading:8920943-Oxidative Stress,
pubmed-meshheading:8920943-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:8920943-Puromycin,
pubmed-meshheading:8920943-Recombinant Proteins,
pubmed-meshheading:8920943-Tetrazolium Salts,
pubmed-meshheading:8920943-Thiazoles,
pubmed-meshheading:8920943-Transfection,
pubmed-meshheading:8920943-Tumor Necrosis Factor-alpha
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pubmed:year |
1996
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pubmed:articleTitle |
Ceramide reproduces late appearance of oxidative stress during TNF-mediated cell death in L929 cells.
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pubmed:affiliation |
Departamento de Biología Celular, Instituto de Fisiología Celular, UNAM, México DF, Mexico.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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