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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1996-12-30
|
| pubmed:databankReference | |
| pubmed:abstractText |
A cDNA of human Gal beta 1,3GalNAc alpha 2,3-sialytransferase (hST3Gal II) which has been known to exhibit much more acceptor substrate preference for glycolipid than for O-linked oligosaccharides of glycoproteins, was isolated from the human liver cDNA library by plaque hybridization using the cDNA of mouse ST3Gal II (mST3Gal II) cloned previously as a probe. Comparative analysis of this cDNA with mST3Gal II indicates 89 and 94% homologies in the nucleotide and amino acid levels, respectively, between the two sequences in the predicted coding region. Northern analysis indicated that the expression of hST3Gal II mRNA is tissue-specific, it being prominent in skeletal muscle and heart, while that in lung and kidney is very low. This enzyme expressed in COS cells showed a similar activity with that of mST3Gal II.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0006-291X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
12
|
| pubmed:volume |
228
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
324-7
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8920913-Amino Acid Sequence,
pubmed-meshheading:8920913-Animals,
pubmed-meshheading:8920913-COS Cells,
pubmed-meshheading:8920913-Cloning, Molecular,
pubmed-meshheading:8920913-Female,
pubmed-meshheading:8920913-Gene Library,
pubmed-meshheading:8920913-Humans,
pubmed-meshheading:8920913-Liver,
pubmed-meshheading:8920913-Mice,
pubmed-meshheading:8920913-Molecular Sequence Data,
pubmed-meshheading:8920913-Open Reading Frames,
pubmed-meshheading:8920913-Organ Specificity,
pubmed-meshheading:8920913-Polymerase Chain Reaction,
pubmed-meshheading:8920913-Pregnancy,
pubmed-meshheading:8920913-Rats,
pubmed-meshheading:8920913-Recombinant Proteins,
pubmed-meshheading:8920913-Sequence Homology, Amino Acid,
pubmed-meshheading:8920913-Sialyltransferases,
pubmed-meshheading:8920913-Swine,
pubmed-meshheading:8920913-Transfection
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Molecular cloning and expression of human Gal beta 1,3GalNAc alpha 2,3-sialytransferase (hST3Gal II).
|
| pubmed:affiliation |
Division of Molecular Glycobiology, Korea Research Institute of Bioscience and Biotechnology, (KRIBB), Taejon, South Korea.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|