Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1997-1-2
pubmed:databankReference
pubmed:abstractText
A novel sulfate-reducing bacterium isolated from fuel-contaminated subsurface soil, strain PRTOL1, mineralizes toluene as the sole electron donor and carbon source under strictly anaerobic conditions. The mineralization of 80% of toluene carbon to CO2 was demonstrated in experiments with [ring-U-14C]toluene; 15% of toluene carbon was converted to biomass and nonvolatile metabolic by-products, primarily the former. The observed stoichiometric ratio of moles of sulfate consumed per mole of toluene consumed was consistent with the theoretical ratio for mineralization of toluene coupled with the reduction of sulfate to hydrogen sulfide. Strain PRTOL1 also transforms o- and p-xylene to metabolic products when grown with toluene. However, xylene transformation by PRTOL1 is slow relative to toluene degradation and cannot be sustained over time. Stable isotope-labeled substrates were used in conjunction with gas chromatography-mass spectrometry to investigate the by-products of toluene and xylene metabolism. The predominant by-products from toluene, o-xylene, and p-xylene were benzylsuccinic acid, (2-methylbenzyl)succinic acid, and 4-methylbenzoic acid (or p-toluic acid), respectively. Metabolic by-products accounted for nearly all of the o-xylene consumed. Enzyme assays indicated that acetyl coenzyme A oxidation proceeded via the carbon monoxide dehydrogenase pathway. Compared with the only other reported toluene-degrading, sulfate-reducing bacterium, strain PRTOL1 is distinct in that it has a novel 16S rRNA gene sequence and was derived from a freshwater rather than marine environment.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-1444436, http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-1575481, http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-1610173, http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-1624475, http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-16347109, http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-16348226, http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-1781729, http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-1814278, http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-1987160, http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-2059037, http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-2244785, http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-2439888, http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-2589921, http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-3105454, http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-7686000, http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-7710331, http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-7993091, http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-8085824, http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-8117084, http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-8193955, http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-8215387, http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-8332524, http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-8439232, http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-8481092, http://linkedlifedata.com/resource/pubmed/commentcorrection/8919780-8588734
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1188-96
pubmed:dateRevised
2010-9-10
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Isolation and characterization of a novel toluene-degrading, sulfate-reducing bacterium.
pubmed:affiliation
Department of Civil Engineering, Stanford University, California 94305-4020, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.