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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
20
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pubmed:dateCreated |
1996-12-18
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pubmed:abstractText |
The quantification of Bcr-Abl transcript numbers in chronic myelogenous leukemia (CML) patients described here uses simultaneous competitive PCR amplification of the target gene (Bcr-Abl) and a reference gene (porphobilinogen deaminase; Pbgd) together with a single composite competitor molecule for both targets based on heterologous sequences. Using this technique, Bcr-Abl transcript numbers could be reproducibly determined even in clinical samples known to harbour poor quality RNA.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
|
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4102-3
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8918822-DNA, Complementary,
pubmed-meshheading:8918822-DNA Primers,
pubmed-meshheading:8918822-Electrophoresis, Agar Gel,
pubmed-meshheading:8918822-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:8918822-Genes, abl,
pubmed-meshheading:8918822-Genetic Vectors,
pubmed-meshheading:8918822-Humans,
pubmed-meshheading:8918822-Hydroxymethylbilane Synthase,
pubmed-meshheading:8918822-Interferons,
pubmed-meshheading:8918822-Leukemia, Myelogenous, Chronic, BCR-ABL Positive,
pubmed-meshheading:8918822-Polymerase Chain Reaction,
pubmed-meshheading:8918822-Transcription, Genetic
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pubmed:year |
1996
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pubmed:articleTitle |
Quantification of Bcr-Abl transcripts in chronic myelogenous leukemia (CML) using standardized, internally controlled, competitive differential PCR (CD-PCR).
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pubmed:affiliation |
Abteilung für Innere Medizin m.S. Hämatologie und Onkologie, Virchow Klinikum, Medizinische Fakultät der Humboldt Universität zu Berlin, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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