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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
1997-1-8
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pubmed:abstractText |
We recently determined that fusion proteins containing tyrosine-based endocytic signals bind to the mu 2 subunit of AP-2, the complex that drives clathrin coat formation and mediates endocytosis from the plasma membrane. Here we analyze the selectivity of peptide recognition by mu 2 and by AP-2 using combinatorial selection methods and surface plasmon resonance. Both mu 2 and AP-2 are shown to interact with various sequences of the form tyrosine-polar-polar-hydrophobic (Yppø) found on receptors that follow the clathrin pathway. The optimal sequence for interaction with mu 2 and with AP-2 has tyrosine as an anchor and prefers arginine at position Y + 2 and leucine at position Y + 3. In contrast, no preferred sequence is detected surrounding the Yppø signal, indicating that recognition of the Yppø endocytic signal does not require a prefolded structure. We conclude that sorting into the endocytic pathway is governed by a surprisingly simple interaction between the mu 2 chain and a tyrosine-containing tetrapeptide sequence.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-1317852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-1379696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-1760844,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-1968060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-2100204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-2257624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-2545438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-2564002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-2905261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-7511210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-7569928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-7588625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-7671305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-7680435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-7680959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-7706244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-7749194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-7781597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-7814360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-7822287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-8076832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-8195226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-8280459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-8342026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-8436587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-8491209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-8509395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-8548289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8918456-8647888
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5789-95
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8918456-Amino Acid Sequence,
pubmed-meshheading:8918456-Clathrin,
pubmed-meshheading:8918456-DNA-Binding Proteins,
pubmed-meshheading:8918456-Endocytosis,
pubmed-meshheading:8918456-Humans,
pubmed-meshheading:8918456-Molecular Sequence Data,
pubmed-meshheading:8918456-Peptide Library,
pubmed-meshheading:8918456-Protein Conformation,
pubmed-meshheading:8918456-Protein Sorting Signals,
pubmed-meshheading:8918456-Recombinant Fusion Proteins,
pubmed-meshheading:8918456-Saccharomyces cerevisiae,
pubmed-meshheading:8918456-Signal Transduction,
pubmed-meshheading:8918456-Transcription Factor AP-2,
pubmed-meshheading:8918456-Transcription Factors,
pubmed-meshheading:8918456-Tyrosine
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pubmed:year |
1996
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pubmed:articleTitle |
Sequence requirements for the recognition of tyrosine-based endocytic signals by clathrin AP-2 complexes.
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pubmed:affiliation |
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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