Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
1996-12-30
pubmed:abstractText
In this work, we report the posttranscriptional addition of poly(A)-rich sequences to mRNA in chloroplasts of higher plants. Several sites in the coding region and the mature end of spinach chloroplast psbA mRNA, which encodes the D1 protein of photosystem II, are detected as polyadenylylated sites. In eukaryotic cells, the addition of multiple adenosine residues to the 3' end of nuclear RNA plays a key role in generating functional mRNAs and in regulating mRNA degradation. In bacteria, the adenylation of several RNAs greatly accelerates their decay. The poly(A) moiety in the chloroplast, in contrast to that in eukaryotic nuclear encoded and bacterial RNAs, is not a ribohomopolymer of adenosine residues, but clusters of adenosines bounded mostly by guanosines and rarely by cytidines and uridines; it may be as long as several hundred nucleotides. Further analysis of the initial steps of chloroplast psbA mRNA decay revealed specific endonuclease cleavage sites that perfectly matched the sites where poly(A)-rich sequences were added. Our results suggest a mechanism for the degradation of psbA mRNA in which endonucleolytic cleavages are followed by the addition of poly(A)-rich sequences to the upstream cleavage products, which target these RNAs for rapid decay.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-1282351, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-1353951, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-1400453, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-1438224, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-16593262, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-1840911, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-1973075, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-2026146, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-2478550, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-2643471, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-3690662, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-4521823, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-7510217, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-7533264, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-7534403, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-7535193, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-7613093, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-7657646, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-7688127, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-7688664, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-7732015, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-7892180, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-8029331, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-8329684, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-8388079, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-8532533, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-8605883, http://linkedlifedata.com/resource/pubmed/commentcorrection/8917603-963026
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
93
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
13398-403
pubmed:dateRevised
2010-9-10
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Addition of destabilizing poly (A)-rich sequences to endonuclease cleavage sites during the degradation of chloroplast mRNA.
pubmed:affiliation
Department of Biology, Technion-Israel Institute of Technology, Haifa, Israel.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't