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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
1996-12-16
|
| pubmed:databankReference | |
| pubmed:abstractText |
A centromere has been isolated from Candida glabrata by functional selection based on the lethality of the SUP11 gene at high copy number. Nucleotide sequence analysis revealed a centromeric structure similar to that of Saccharomyces cerevisiae: the two highly conserved elements CDEI (8 bp) and CDEIII (26 bp) are separated by a 79-bp A+T-rich element, CDEII. Three centromere-bearing plasmid vectors with different selection markers have been constructed. These plasmids were highly stable in mitosis (< 1% loss rate per generation) and exist in one or two copies per cell.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0378-1119
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
10
|
| pubmed:volume |
175
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
105-8
|
| pubmed:dateRevised |
2006-4-21
|
| pubmed:meshHeading |
pubmed-meshheading:8917084-Base Sequence,
pubmed-meshheading:8917084-Candida,
pubmed-meshheading:8917084-Centromere,
pubmed-meshheading:8917084-Conserved Sequence,
pubmed-meshheading:8917084-DNA, Fungal,
pubmed-meshheading:8917084-Genetic Vectors,
pubmed-meshheading:8917084-Molecular Sequence Data,
pubmed-meshheading:8917084-Sequence Analysis, DNA
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Isolation of a Candida glabrata centromere and its use in construction of plasmid vectors.
|
| pubmed:affiliation |
Department of Mycology, Nippon Roche Research Center, Kanagawa, Japan. kunio.kitada@roche.com
|
| pubmed:publicationType |
Journal Article
|