Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1996-12-17
pubmed:abstractText
Accelerated coronary atherosclerosis in cardiac allografts is the major limiting factor for long-term survival after heart transplantation. There is growing evidence that activation of the coagulation mechanism is involved in the development of transplant atherosclerosis. Tissue factor (TF) expression by cells of the monocyte/macrophage system may represent an important mechanism underlying the fibrin deposition in the affected vessels. In the present study, we investigated the effect of cyclosporine A (CsA) on the lipopolysaccharide (LPS)-induced procoagulant activity (PCA) in human monocytes/macrophages. CsA exerted a dose-dependent inhibitory effect on LPS-induced monocyte/macrophage PCA, which was identified as TF activity based on functional and immunologic characterization. As shown by reverse transcriptase-polymerase chain reaction, CsA reduced the transcription of the TF gene in LPS-stimulated monocytes/macrophages. Electrophoretic mobility shift assay showed that CsA inhibited the LPS-induced activation of the nuclear factor kappa B (NF-kappa B). As shown by Western blot analysis, CsA treatment decreased the nuclear translocation of NF-kappa B, thereby suggesting the mechanism for the inhibitory effect of CsA on TF induction. Hence, a nonimmunologic effect of CsA may contribute to its successful use in transplant medicine.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3837-45
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:8916948-Biological Transport, pubmed-meshheading:8916948-Blood Coagulation Factors, pubmed-meshheading:8916948-Cell Nucleus, pubmed-meshheading:8916948-Cells, Cultured, pubmed-meshheading:8916948-Coronary Artery Disease, pubmed-meshheading:8916948-Cyclosporine, pubmed-meshheading:8916948-Depression, Chemical, pubmed-meshheading:8916948-Gene Expression Regulation, pubmed-meshheading:8916948-Heart Transplantation, pubmed-meshheading:8916948-Humans, pubmed-meshheading:8916948-Lipopolysaccharides, pubmed-meshheading:8916948-Macrophages, pubmed-meshheading:8916948-Monocytes, pubmed-meshheading:8916948-NF-kappa B, pubmed-meshheading:8916948-Postoperative Complications, pubmed-meshheading:8916948-Protein Binding, pubmed-meshheading:8916948-Thromboplastin, pubmed-meshheading:8916948-Transcription, Genetic
pubmed:year
1996
pubmed:articleTitle
Cyclosporine a inhibits tissue factor expression in monocytes/macrophages.
pubmed:affiliation
Department of Internal Medicine, Justus-Liebig-University Giessen, Germany.
pubmed:publicationType
Journal Article