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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1997-2-6
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pubmed:databankReference | |
pubmed:abstractText |
Two cDNA clones, encoding mink Ig gamma chains were characterized. The pIGG47 clone contains a part of the leader segment, VDJ and C regions, and pIGG14 contains a part of the J and a complete C region. The clones differ by only four nucleotides in the C region, and they most probably represent allelic variants of the same gene. The V gene segment of pIGG47 was found to be highly similar to human VHIII subgroup sequences; there was 86-87% similarity for the whole V gene segment and 91% for the VHIII specific regions (codons 65-87). Southern blot analysis demonstrated that a high proportion of mink VH genes is VHIII related. The V gene segment used as a probe revealed 19-23 bands in mink DNA under stringent conditions. This is in agreement with our previous data showing that a high proportion of mink Ig contains an 'alternative' binding site for protein A, a feature common to VHIII-related molecules. According to Southern blot analysis there may be 5-7 C gamma genes at the mink IgH locus.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Heavy Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Variable Region,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin gamma-Chains
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pubmed:status |
MEDLINE
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pubmed:issn |
0145-305X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
231-40
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8915626-Amino Acid Sequence,
pubmed-meshheading:8915626-Animals,
pubmed-meshheading:8915626-Base Sequence,
pubmed-meshheading:8915626-Cloning, Molecular,
pubmed-meshheading:8915626-DNA, Complementary,
pubmed-meshheading:8915626-Genes, Immunoglobulin,
pubmed-meshheading:8915626-Humans,
pubmed-meshheading:8915626-Immunoglobulin Heavy Chains,
pubmed-meshheading:8915626-Immunoglobulin Variable Region,
pubmed-meshheading:8915626-Immunoglobulin gamma-Chains,
pubmed-meshheading:8915626-Mice,
pubmed-meshheading:8915626-Mink,
pubmed-meshheading:8915626-Molecular Sequence Data,
pubmed-meshheading:8915626-Rabbits,
pubmed-meshheading:8915626-Spleen
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pubmed:articleTitle |
Structure of mink immunoglobulin gamma chain cDNA.
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pubmed:affiliation |
Institute of Cytology and Genetics, Novosibirsk, Russia.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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