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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1997-3-11
|
| pubmed:abstractText |
We report a simple and effective means for delivering Morpholino antisense oligos into the cytosol of cultured anchorage-dependent animal cells. This method, referred to as scrape-loading, is carried out in a matter of seconds, uses a common inexpensive laboratory implement, and has minimal detrimental impact on the cells. Using this delivery method, a Morpholino oligo present at 0.1 microM and 1 microM in the extracellular medium inhibited its targeted genetic sequence within cultured Hela cells at levels of 56% and 85%, respectively. Lack of inhibition by two control Morpholino oligos at concentrations up to 3 microM indicates good sequence specificity by this structural type. Also described is a test system for simple, rapid, and sensitive quantitation of antisense activity in cultured cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
1087-2906
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
6
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
169-75
|
| pubmed:dateRevised |
2006-5-1
|
| pubmed:meshHeading |
pubmed-meshheading:8915501-Animals,
pubmed-meshheading:8915501-Base Sequence,
pubmed-meshheading:8915501-Cell Line,
pubmed-meshheading:8915501-Cytological Techniques,
pubmed-meshheading:8915501-Cytoplasm,
pubmed-meshheading:8915501-Enzyme Induction,
pubmed-meshheading:8915501-HeLa Cells,
pubmed-meshheading:8915501-Humans,
pubmed-meshheading:8915501-Luciferases,
pubmed-meshheading:8915501-Molecular Sequence Data,
pubmed-meshheading:8915501-Morpholines,
pubmed-meshheading:8915501-Oligonucleotides, Antisense,
pubmed-meshheading:8915501-Plasmids,
pubmed-meshheading:8915501-Rats,
pubmed-meshheading:8915501-Sensitivity and Specificity,
pubmed-meshheading:8915501-Time Factors,
pubmed-meshheading:8915501-Transfection,
pubmed-meshheading:8915501-Trypsin
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
A simple method for delivering morpholino antisense oligos into the cytoplasm of cells.
|
| pubmed:affiliation |
ANTIVIRALS Inc., Corvallis, OR 97333, USA.
|
| pubmed:publicationType |
Journal Article
|