Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1997-2-18
pubmed:abstractText
The Western blot (immunoblot) patterns of 56 serum specimens, all examined previously by the microimmunofluorescence (MIF) test for species-specific Chlamydia antibodies, were analyzed. Predominant specific-antibody activity was directed to the 170-, 155-, 145-, 120-, 115-, 100-, 57-, and 38-kDa proteins of Chlamydia trachomatis and to the 175-, 130-, 110-, 98-, and 30-kDa proteins of Chlamydia pneumoniae. All of these antigens appeared to be species specific. The reactivity with 90-, 80-, 75-, 62- or 60-, and 55-kDa proteins and the major outer membrane protein appeared to be genus specific. Fourteen serum samples which had identical titers of immunoglobulin G as determined by the MIF test were investigated by Western blotting. We found that nine serum samples had anti-C. trachomatis protein profiles and two had anti-C. pneumoniae protein profiles. The double seropositivity observed by MIF corresponded with cross-reactivity to genus-specific antibodies. As for the three remaining serum specimens, we observed identical protein profiles for C. trachomatis and C. pneumoniae, confirming the double seropositivity experienced with the MIF test. Western blotting can differentiate between specific reactions and interfering noise from other, partly cross-reacting chlamydial species.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-1581191, http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-1608116, http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-1624880, http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-2029495, http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-2181028, http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-2205652, http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-2205784, http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-2294048, http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-2294060, http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-2380354, http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-2456851, http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-3519787, http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-3546521, http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-3793227, http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-388439, http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-4915925, http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-5154904, http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-51883, http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-5962951, http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-6266278, http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-6425219, http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-8187792, http://linkedlifedata.com/resource/pubmed/commentcorrection/8914762-8370753
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1071-412X
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
706-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Limits of the microimmunofluorescence test and advantages of immunoblotting in the diagnosis of chlamydiosis.
pubmed:affiliation
Laboratoire de Bactériologie-Immunologie Générale, CHU Nord, France.
pubmed:publicationType
Journal Article, Comparative Study