Linked Life Data

8913868

Source:http://linkedlifedata.com/resource/pubmed/id/8913868

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-3-3
pubmed:abstractText
We have carried out immunofluorescence labelling of two human cell types, HeLa cells and peripheral blood lymphocytes, prepared by several different fixation/permeabilization protocols using a variety of antibodies against DNA Topoisomerase II (Topo II). We have found that the distribution of Topo II alpha was overall similar doing interphase and mitosis to that previously reported, regardless of antibody and of sample preparation. On the other land, the interphase distribution of Topo II beta was quite variable, depending both on the antibody and on the method used to prepare the sample. Our interpretation of the data is that, like Topo II alpha, Topo II beta is primarily a nucleoplasmic protein, but that unlike Topo II alpha, small amounts are also associated with intranucleolar chromatin.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0730-2312
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
63
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
162-73
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Is DNA topoisomerase II beta a nucleolar protein?
pubmed:affiliation
Department of Biology, Carleton University, Ottawa ON, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't