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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1996-12-26
|
| pubmed:abstractText |
Hepatic cytochromes P450 are known to be down-regulated by cytokines, lipopolysaccharide, Gram-positive bacteria, and viruses. Little is known, however, about the regulation by inflammation of cytochromes P450 in other tissues. We have found that lipopolysaccharide and interleukin-1 beta stimulate the expression of catalytically active CYP2E1 (but not CYP1A1 or CYP2B) up to 7-fold in rat brain primary cortical glial cultures. The induction reached a maximum after 24 hr and was accompanied by an increase in CYP2E1 mRNA. Chlormethiazole, a specific inhibitor of hepatic CYP2E1 transcription, completely inhibited the induction of CYP2E1 at the mRNA and enzyme levels. Immunofluorescence studies showed CYP2E1 to be expressed in a subset of astrocytes in the lipopolysaccharide-stimulated cortical glial cultures. Using a model of global ischemic injury in the gerbil, we found CYP2E1 to be induced in vivo in astrocytes in the inflammatory phase, 1-3 weeks after the lesion. Likewise, CYP2E1 was induced in the rat cortex 1 week after a focal ischemic injury. Our results suggest tissue-specific regulation of CYP2E1 by inflammatory factors and that CYP2E1 may play a role in astrocytes during inflammation in the brain.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chlormethiazole,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorzoxazone,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 CYP2E1,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0026-895X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
50
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1065-72
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8913336-Animals,
pubmed-meshheading:8913336-Astrocytes,
pubmed-meshheading:8913336-Brain Ischemia,
pubmed-meshheading:8913336-Cells, Cultured,
pubmed-meshheading:8913336-Cerebral Cortex,
pubmed-meshheading:8913336-Chlormethiazole,
pubmed-meshheading:8913336-Chlorzoxazone,
pubmed-meshheading:8913336-Cytochrome P-450 CYP2E1,
pubmed-meshheading:8913336-Enzyme Induction,
pubmed-meshheading:8913336-Enzyme Inhibitors,
pubmed-meshheading:8913336-Gerbillinae,
pubmed-meshheading:8913336-Hydroxylation,
pubmed-meshheading:8913336-Inflammation,
pubmed-meshheading:8913336-Interleukin-1,
pubmed-meshheading:8913336-Kinetics,
pubmed-meshheading:8913336-Lipopolysaccharides,
pubmed-meshheading:8913336-Male,
pubmed-meshheading:8913336-RNA, Messenger,
pubmed-meshheading:8913336-Rats,
pubmed-meshheading:8913336-Rats, Sprague-Dawley
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Induction of cytochrome P450 2E1 expression in rat and gerbil astrocytes by inflammatory factors and ischemic injury.
|
| pubmed:affiliation |
Department of Medical Biochemistry and Biophysics, Karolinska institute, Stockholm, Sweden.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|