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rdf:type |
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lifeskim:mentions |
umls-concept:C0025219,
umls-concept:C0032005,
umls-concept:C0040648,
umls-concept:C0085828,
umls-concept:C0205263,
umls-concept:C0229530,
umls-concept:C0449432,
umls-concept:C1179435,
umls-concept:C1524073,
umls-concept:C1548799,
umls-concept:C1705248,
umls-concept:C1744525
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pubmed:issue |
1
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pubmed:dateCreated |
1996-12-20
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pubmed:abstractText |
In ovine pars tuberalis cells which express high affinity Mel 1a melatonin receptors, the ability of melatonin to directly stimulate or inhibit AP-1 transcription factor gene expression was studied. Effects of melatonin upon mRNA expression by forskolin, serum and IGF-1 were also investigated. Northern analysis showed melatonin had no direct stimulatory nor inhibitory effect upon transcription or translation. Melatonin was able to significantly inhibit forskolin-stimulated induction of c-fos and jun B mRNA whilst forskolin had no effect upon c-jun or jun D. Induction of c-Fos translation by forskolin was also inhibited by melatonin. Serum induced c-fos and c-jun, but melatonin was unable to affect these changes. Similarly IGF-1 stimulated c-fos and melatonin had no effect upon this induction. From these results it can be concluded that melatonin has no independent effects on expression of the AP-1 genes, rather its primary function is to inhibit cell activities through cyclic AMP-dependent routes of gene activation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0303-7207
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
123
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
71-80
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8912813-Animals,
pubmed-meshheading:8912813-Base Sequence,
pubmed-meshheading:8912813-Blotting, Northern,
pubmed-meshheading:8912813-Cells, Cultured,
pubmed-meshheading:8912813-Female,
pubmed-meshheading:8912813-Forskolin,
pubmed-meshheading:8912813-Gene Expression,
pubmed-meshheading:8912813-Genes, jun,
pubmed-meshheading:8912813-In Situ Hybridization,
pubmed-meshheading:8912813-Insulin-Like Growth Factor I,
pubmed-meshheading:8912813-Male,
pubmed-meshheading:8912813-Melatonin,
pubmed-meshheading:8912813-Oligonucleotide Probes,
pubmed-meshheading:8912813-Pituitary Gland, Anterior,
pubmed-meshheading:8912813-Proto-Oncogene Proteins c-jun,
pubmed-meshheading:8912813-RNA, Messenger,
pubmed-meshheading:8912813-Sheep,
pubmed-meshheading:8912813-Transcription, Genetic,
pubmed-meshheading:8912813-Transcription Factor AP-1
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pubmed:year |
1996
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pubmed:articleTitle |
Melatonin suppresses the induction of AP-1 transcription factor components in the pars tuberalis of the pituitary.
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pubmed:affiliation |
Rowett Research Institute, Aberdeen, Scotland, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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