Linked Life Data

8912670

Source:http://linkedlifedata.com/resource/pubmed/id/8912670

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1996-12-26
pubmed:abstractText
A method is described for the production of detailed maps of intralobular variations of hepatocyte function and metabolite concentrations, based on variable destruction by digitonin of the lobule from the centrilobular direction. Instead of the conventional approach, in which isolated hepatocytes are then prepared and studied in suspension, perfusion is continued after digitonin treatment and the function of the unaffected lobular remnants is determined, or mean metabolite concentrations are measured by 31P-NMR. These measurements are plotted against the degree of destruction, determined precisely after each study by automated quantitative histomorphometry. These plots are transformed into curves of the function or metabolite concentration of nominal single cells at any point along the radius of the lobule. Gluconeogenesis from lactate remained stable, although reduced, even after 85-90% lobular destruction, predominated periportally and disappeared by 50% along the radius of the lobule. In 31P-NMR studies, employing 1.5 mM lactate as substrate, narrowing of the intracellular P1 resonance was observed as digitonin destruction increased; this was attributed to a decrease in the intralobular heterogeneity of the intracellular pH, which fell from approx. 7.9 to < 7.4 along the first 16% of the lobular radius (from the periportal end) and to < 7.3 in the remainder of the lobule. The ATP concentration rose, and then fell, along the radius of the lobule in a centripetal direction. The method is potentially generally applicable to a wide range of hepatocellular functions and to the measurement of metabolite concentrations, most conveniently those susceptible to estimation by NMR.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8912670, http://linkedlifedata.com/resource/pubmed/commentcorrection/8912670-1017801, http://linkedlifedata.com/resource/pubmed/commentcorrection/8912670-2106931, http://linkedlifedata.com/resource/pubmed/commentcorrection/8912670-2178605, http://linkedlifedata.com/resource/pubmed/commentcorrection/8912670-2241921, http://linkedlifedata.com/resource/pubmed/commentcorrection/8912670-2512912, http://linkedlifedata.com/resource/pubmed/commentcorrection/8912670-2664826, http://linkedlifedata.com/resource/pubmed/commentcorrection/8912670-2807995, http://linkedlifedata.com/resource/pubmed/commentcorrection/8912670-2994630, http://linkedlifedata.com/resource/pubmed/commentcorrection/8912670-3143359, http://linkedlifedata.com/resource/pubmed/commentcorrection/8912670-3223900, http://linkedlifedata.com/resource/pubmed/commentcorrection/8912670-3814074, http://linkedlifedata.com/resource/pubmed/commentcorrection/8912670-3965451, http://linkedlifedata.com/resource/pubmed/commentcorrection/8912670-3977871, http://linkedlifedata.com/resource/pubmed/commentcorrection/8912670-4038253, http://linkedlifedata.com/resource/pubmed/commentcorrection/8912670-5564306, http://linkedlifedata.com/resource/pubmed/commentcorrection/8912670-7487941, http://linkedlifedata.com/resource/pubmed/commentcorrection/8912670-8515054
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
319 ( Pt 2)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
377-83
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
A method for determination in situ of variations within the hepatic lobule of hepatocyte function and metabolite concentrations.
pubmed:affiliation
Medical Unit, St. Bartholomew's, U.K.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't