Linked Life Data

8911667

Source:http://linkedlifedata.com/resource/pubmed/id/8911667

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-2-18
pubmed:abstractText
We investigated the temporo-spatial expression of astrocyte glial fibrillary acidic protein (gfap) and sulfated glycoprotein 2 (sgp-2) mRNAs in comparison to 70-kDa heat shock protein (hsp70) mRNA by in situ hybridisation in rats subjected to permanent occlusion of the middle cerebral artery (MCA). Gfap mRNA started to increase in the cingulate cortex of the lesioned hemisphere 6 h after MCA occlusion and gradually spread over the lateral part of the ipsilateral cortex and the striatum from 12 h to 3 days, peaking at 3 days after MCA occlusion. Gfap mRNA also increased in the contralateral cingulate cortex and corpus callosum at 12 and 24 h. Hsp70 mRNA increased markedly in the ipsilateral cortex adjacent to the ischemic lesion, and slightly within the lesion area from 3 to 24 h and disappeared after 3 days. By 7 days, gfap and sgp-2 mRNAs were increased markedly in the peri-infarct area, and in the ipsilateral thalamus parallel with the delayed neuronal damage, whereas the widespread increase of gfap mRNA in the ipsilateral hemisphere declined. Post-occlusion treatment with the glutamate receptor antagonists MK-801 and NBQX slightly attenuate the induction of gfap but did not qualitatively affect the topical expression pattern. Within the cingulate cortex MK-801 treatment resulted in a significant decrease of the signal intensity at all survival times, reflecting most likely an attenuation of lesion-induced spreading depression like depolarization waves by MK-801. The area of hsp70 expression was reduced by both MK-801 and NBQX, most likely reflecting the decrease of the lesion area by both treatment regimens. Our study thus revealed an early and widespread increase of gfap mRNA in the non-ischemic area including the contralateral hemisphere starting between 3 and 6 h, and a delayed circumscribed expression in the peri-infarct border zone after 1 week. Comparison with the expression of hsp70 mRNA suggests that the absence of an early gfap mRNA induction in the peri-lesion zone reflects an impairment of astrocytic function which may be of importance for infarct growth during the early evolution of the pathological process.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
7
pubmed:volume
735
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
285-97
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8911667-Animals, pubmed-meshheading:8911667-Astrocytes, pubmed-meshheading:8911667-Brain Ischemia, pubmed-meshheading:8911667-Clusterin, pubmed-meshheading:8911667-Dizocilpine Maleate, pubmed-meshheading:8911667-Electroencephalography, pubmed-meshheading:8911667-Excitatory Amino Acid Antagonists, pubmed-meshheading:8911667-Glial Fibrillary Acidic Protein, pubmed-meshheading:8911667-Glycoproteins, pubmed-meshheading:8911667-HSP70 Heat-Shock Proteins, pubmed-meshheading:8911667-In Situ Hybridization, pubmed-meshheading:8911667-Male, pubmed-meshheading:8911667-Molecular Chaperones, pubmed-meshheading:8911667-Quinoxalines, pubmed-meshheading:8911667-RNA, Messenger, pubmed-meshheading:8911667-Rats, pubmed-meshheading:8911667-Rats, Inbred F344, pubmed-meshheading:8911667-Time Factors
pubmed:year
1996
pubmed:articleTitle
Monitoring the temporal and spatial activation pattern of astrocytes in focal cerebral ischemia using in situ hybridization to GFAP mRNA: comparison with sgp-2 and hsp70 mRNA and the effect of glutamate receptor antagonists.
pubmed:affiliation
Max-Planck-Institute for Neurological Research, Department of Experimental Neurology, Cologne, Germany.
pubmed:publicationType
Journal Article, Comparative Study