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rdf:type |
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lifeskim:mentions |
umls-concept:C0017890,
umls-concept:C0027882,
umls-concept:C0033640,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0036037,
umls-concept:C0036751,
umls-concept:C0205095,
umls-concept:C0871261,
umls-concept:C1627358,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C2349975,
umls-concept:C2911692
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pubmed:dateCreated |
1997-2-19
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pubmed:abstractText |
1. The modulatory effect of serotonin (5-hydroxytryptamine, 5-HT), on the glycine (Gly) response was investigated in neurones acutely dissociated from the rat sacral dorsal commissural nucleus (SDCN) using a nystatin-perforated patch recording configuration. 2. 5-HT potentiated the 10(-5) M Gly-induced Cl- current (IGly) in a concentration-dependent manner without changing the reversal potential of the Gly response or the affinity of Gly to its receptor. 3. alpha-Methyl-5-HT mimicked and ketanserine blocked the 5-HT action on IGly, thus indicating the 5-HT2 receptor-mediated enhancement. 4. Phorbol-12-myristate-13-acetate and 1-oleoyl-2-acetylglycerol potentiated IGly. The subsequent application of 5-HT slightly increase IGly. Chelerythrine blocked the enhancement of IGly by 5-HT, thus suggesting the involvement of protein kinase C (PKC) in the pathway of 5-HT action on IGly. 5. Pertussis toxin (IAP) treatment did not block the facilitatory effect of 5-HT on IGly. 6. BAPTA AM did not disturb the 5-HT-induced potentiation of IGly, thus suggesting that [Ca2+]i is not involved in the 5-HT effect. 7. In conclusion, activation of a 5-HT2 receptor coupled to an IAP-insensitive G-protein increases intracellular diacylglycerol (DAG) formation. The accumulation of DAG also increases the Ca(2+)-independent PKC activity, thus resulting in the potentiation of the Gly response in the SDCN neurones.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-1380014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-1707501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-1717872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-1722365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-2147117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-2172840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-2370323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-2709447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-2887235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-3443977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-3998792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-4032293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-6142933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-6196383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-6206440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-7504109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-7538569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-7561053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-7583309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-7658366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-7682348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-7696616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-7702566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-7730987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-7751909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-7756615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-7820676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-7953766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-7984266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-8294452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-8350989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-8484714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-8492147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8910232-8592640
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Chelating Agents,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Glycine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin Receptor Agonists
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
496 ( Pt 2)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
491-501
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:8910232-Animals,
pubmed-meshheading:8910232-Calcium,
pubmed-meshheading:8910232-Chelating Agents,
pubmed-meshheading:8910232-Electrophysiology,
pubmed-meshheading:8910232-GTP-Binding Proteins,
pubmed-meshheading:8910232-Neurons,
pubmed-meshheading:8910232-Patch-Clamp Techniques,
pubmed-meshheading:8910232-Protein Kinase C,
pubmed-meshheading:8910232-Rats,
pubmed-meshheading:8910232-Rats, Wistar,
pubmed-meshheading:8910232-Receptors, Glycine,
pubmed-meshheading:8910232-Receptors, Serotonin,
pubmed-meshheading:8910232-Serotonin,
pubmed-meshheading:8910232-Serotonin Antagonists,
pubmed-meshheading:8910232-Serotonin Receptor Agonists,
pubmed-meshheading:8910232-Signal Transduction,
pubmed-meshheading:8910232-Spinal Cord
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pubmed:year |
1996
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pubmed:articleTitle |
Protein kinase C-mediated enhancement of glycine response in rat sacral dorsal commissural neurones by serotonin.
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pubmed:affiliation |
Department of Physiology, Kyushu University Faculty of Medicine, Fukuoka, Japan.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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