Linked Life Data

8909786

Source:http://linkedlifedata.com/resource/pubmed/id/8909786

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1997-3-4
pubmed:abstractText
The electrical membrane properties and ionic currents of cultured identified goldfish gonadotrophs were characterized using whole-cell patch-clamp recordings. Under current-clamp recording conditions, goldfish gonadotrophs had a resting membrane potential of approximately -60 mV and an input resistance of 8.2 +/- 1.6 G omega. In response to depolarizing current injections, one or more action potentials could be induced from resting membrane potential or more hyperpolarized holding potentials. In addition, some cells exhibited spontaneous action potential activity. Under voltage-clamp recording conditions, several outward and inward currents were isolated and characterized using pharmacological and ionic substitution studies. The outward currents included a fast-activating, transient current and an inactivation-resistant current similar to IA-type and delayed rectifier currents, respectively. Unlike mammalian gonadotrophs, apamin-sensitive K+ currents were not detected. Inward currents included a fast-activating and inactivating Na+ current and a high-voltage activated, dihydropyridine-sensitive Ca2+ current. The value for half-maximal steady-state inactivation of the Na+ and Ca2+ currents was -50.8 and -16.7 mV, respectively, indicating that a significant proportion of both Na+ and Ca2+ channels are available for activation at the resting membrane potential of these cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0008-4212
pubmed:author
pubmed:issnType
Print
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
729-43
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Electrical membrane properties and ionic currents in cultured goldfish gonadotrophs.
pubmed:affiliation
Department of Biological Sciences, University of Alberta, Edmonton, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't