Linked Life Data

8904428

Source:http://linkedlifedata.com/resource/pubmed/id/8904428

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1997-2-25
pubmed:abstractText
A new enzyme immunoassay (EIA), the Cobas Core Anti-HIV-1/HIV-2 EIA DAGS (also referred to as Roche DAGS), for the detection of antibodies to human immunodeficiency virus type 1 (HIV-1) and HIV-2 was evaluated in four centers. The assay is based on the double-antigen sandwich (DAGS) format, which enables the detection of all classes of antibodies. The antigens consist of recombinant proteins in their native conformation and of synthetic peptides. Of a total of 5,836 negative serum samples, including 95 samples likely to produce false reactivities, 6 were false positive, resulting in a specificity of 99.9%. None of 35 sera that were from noninfected individuals but contained p24-cross-reacting antibodies as revealed by Western blot (immunoblot) analysis were reactive by the Roche DAGS assay. In samples from individuals infected with HIV-1 group M (n = 499) and HIV-2 (n = 200), the sensitivity of the assay was 100%. Although containing antigens with sequences from subtype B only, the assay was also able to correctly identify with high optical density/cutoff ratios samples from subjects infected with HIV-1 subtype O (n = 10). In 17 of 19 seroconversion panels tested, the assay detected the presence of HIV-1 antibodies as early as another sandwich EIA. Eight of these panels were also analyzed by an indirect second-generation assay, which detected antibodies 2 to 10 days later than did the DAGS assay under evaluation. The excellent specificity and sensitivity of the new Cobas Core Anti-HIV-1/HIV-2 EIA DAGS are the result of the DAGS format as well as of the native, naturally folded form of the recombinant protein used as the gag antigen.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8904428-1332725, http://linkedlifedata.com/resource/pubmed/commentcorrection/8904428-1356186, http://linkedlifedata.com/resource/pubmed/commentcorrection/8904428-1401002, http://linkedlifedata.com/resource/pubmed/commentcorrection/8904428-1572966, http://linkedlifedata.com/resource/pubmed/commentcorrection/8904428-2198964, http://linkedlifedata.com/resource/pubmed/commentcorrection/8904428-2733126, http://linkedlifedata.com/resource/pubmed/commentcorrection/8904428-3067617, http://linkedlifedata.com/resource/pubmed/commentcorrection/8904428-3128992, http://linkedlifedata.com/resource/pubmed/commentcorrection/8904428-7583924, http://linkedlifedata.com/resource/pubmed/commentcorrection/8904428-7679241, http://linkedlifedata.com/resource/pubmed/commentcorrection/8904428-7848605, http://linkedlifedata.com/resource/pubmed/commentcorrection/8904428-7898241, http://linkedlifedata.com/resource/pubmed/commentcorrection/8904428-7910884, http://linkedlifedata.com/resource/pubmed/commentcorrection/8904428-7975221, http://linkedlifedata.com/resource/pubmed/commentcorrection/8904428-8107219, http://linkedlifedata.com/resource/pubmed/commentcorrection/8904428-8107220, http://linkedlifedata.com/resource/pubmed/commentcorrection/8904428-8235661, http://linkedlifedata.com/resource/pubmed/commentcorrection/8904428-8507411
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
634-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Multicenter evaluation of new double-antigen sandwich enzyme immunoassay for measurement of anti-human immunodeficiency virus type 1 and type 2 antibodies.
pubmed:affiliation
Division d'Immunologie et Allergie, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
pubmed:publicationType
Journal Article, Multicenter Study