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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1997-1-30
|
| pubmed:abstractText |
We describe a quantitative polymerase chain reaction (PCR) technique with a colorimetric read-out, which enables quantitation of various sequences on a single microplate within one day. Using the quantitation of human IL10 and beta-actin transcripts, we show that this technique provides better reproducibility, as results are derived from a series of PCRs made with various amounts of standard. This also enables the control of the consistency of the data and elimination of some artifactual results.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0923-2494
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
147
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
403-6
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8903107-Actins,
pubmed-meshheading:8903107-Colorimetry,
pubmed-meshheading:8903107-Humans,
pubmed-meshheading:8903107-Interleukin-10,
pubmed-meshheading:8903107-Polymerase Chain Reaction,
pubmed-meshheading:8903107-Reproducibility of Results,
pubmed-meshheading:8903107-Sensitivity and Specificity
|
| pubmed:articleTitle |
Quantitative PCR with colorimetric detection.
|
| pubmed:affiliation |
Unité de Biologie Moléculaire du Gène, INSERM U277, Institut Pasteur, Paris.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|