Linked Life Data

8902197

Source:http://linkedlifedata.com/resource/pubmed/id/8902197

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-2-28
pubmed:abstractText
Free radicals are thought to be involved in the pathogenesis of many disease states such as diabetes, cataractogenesis, cancer, and aging. Involvement of free radicals in most of these disorders, especially diabetic cataractogenesis, however, is derived from indirect evidence only. The purpose of this study was to directly detect the free radicals generated in lens homogenate incubated under hyperglycemic conditions. We investigated the generation of free radicals in lens homogenates incubated with 5.5 or 50 mM glucose containing the spin trap 5,5'-dimethyl 1-pyroline-N-oxide. The spin adducts were identified by electron spin resonance (ESR) spectroscopy and modulation of the levels of the spin adducts was achieved by including various antioxidants and metal chelators. Increased ESR signals were observed in the homogenates incubated with 50 mM glucose compared to those incubated with 5.5 mM glucose. The results show that the principal spin adduct has hyperfine splitting constants of a(N) = 1.41 +/- 0.01 mt and a(N) = 2.02 +/- 0.02 mt, indicating that these signals are due to carbon-centered free radicals. Superoxide dismutase, catalase, Trolox, and diethylenetriamine pentaacetic acid inhibited the signals while EDTA enhanced them. Based upon the results obtained by the inclusion of various modulators, it is concluded that the generation of the free radicals is mediated by a metal-derived Fenton type reaction.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1077-3150
pubmed:author
pubmed:issnType
Print
pubmed:volume
59
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
68-71
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Glucose-dependent formation of free radical species in lens homogenate.
pubmed:affiliation
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77555, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't