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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1996-12-10
|
| pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X99352,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X99353,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X99354,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X99355,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X99356,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X99357,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X99358,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X99359,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X99360,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X99361,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X99362,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X99363,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X99364,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X99365,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X99366,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X99367
|
| pubmed:abstractText |
We analyzed the rheumatoid factors (RF) produced by Epstein-Barr virus-transformed monoclonal B cells established from four patients with rheumatoid arthritis (RA), three individuals with a history of Mycobacterium tuberculosis (TB) and four normal controls (NI). Fifty-eight RF were analyzed for specific activity (international units-RF/microgram) for the Fc part of IgG and their interaction with tetanus toxoid (TT) and DNA (polyspecificity). Furthermore, we sequenced the V-D-J heavy chain region of 16 (9TB-/7RA-) RF. Significant differences were observed between the NI-RF and the TB- and RA-RF. While the RF repertoire of normal individuals comprised of low-avidity RF of which the majority (15/17) were polyspecific, more than half of the TB- and RA-RF were monoreactive. Furthermore, the monospecific TB- and RA-RF were of significantly higher avidity than the NI-RF (RA > TB > > NI). With respect to polyspecificity specificity, the RF in the three groups were comparable: the interaction with DNA, TT as well as with Fc was inhibited either by an increase of the ionic strength to 0.3-0.5 M NaCl or by addition of the polyanion dextran sulfate, indicating that the antibodies interacted with similar anionic epitopes shared by the three antigens. Analysis of the V-D-J heavy chain regions showed significant differences between the respective RF. The salt-sensitive binding was highly correlated with the presence of arginine in the complementarity-determining region 3 (CDR3). Furthermore, whereas the polyspecific RF consisted predominantly of germ-line encoded antibodies, the genes of the monospecific RA/TB-RF were somatically mutated (RA > TB). It is therefore likely that maturation of RF can be initiated by chronic infections and that monospecific, somatically mutated RF are not a unique characteristic of autoimmune diseases.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0014-2980
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
26
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2480-6
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8898963-Adolescent,
pubmed-meshheading:8898963-Adult,
pubmed-meshheading:8898963-Aged,
pubmed-meshheading:8898963-Amino Acid Sequence,
pubmed-meshheading:8898963-Anions,
pubmed-meshheading:8898963-Antibody Specificity,
pubmed-meshheading:8898963-Arthritis, Rheumatoid,
pubmed-meshheading:8898963-Base Sequence,
pubmed-meshheading:8898963-Epitopes,
pubmed-meshheading:8898963-Female,
pubmed-meshheading:8898963-Genes, Immunoglobulin,
pubmed-meshheading:8898963-Humans,
pubmed-meshheading:8898963-Male,
pubmed-meshheading:8898963-Middle Aged,
pubmed-meshheading:8898963-Molecular Sequence Data,
pubmed-meshheading:8898963-Rheumatoid Factor,
pubmed-meshheading:8898963-Tuberculosis, Miliary,
pubmed-meshheading:8898963-Tuberculosis, Osteoarticular
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Comparison of rheumatoid factors of rheumatoid arthritis patients, of individuals with mycobacterial infections and of normal controls: evidence for maturation in the absence of an autoimmune response.
|
| pubmed:affiliation |
Department of Medicine, Hôpital cantonal universitaire de Genève, Switzerland.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|