Linked Life Data

8896817

Source:http://linkedlifedata.com/resource/pubmed/id/8896817

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1997-2-18
pubmed:abstractText
In order to clarify the role of the hypothalamic cholinergic system in the regulation of peripheral glucose metabolism, we investigated hypothalamic cholinergic activity after administration of 2-deoxyglucose (2-DG). Intravenous administration of 2-DG (500 mg/kg) caused marked hyperglycemia; the level of plasma glucose increased by 2.1 times over the initial levels at 20 min. For evaluation of the cholinergic activity, we analyzed extracellular levels of acetylcholine (ACh) and choline using brain microdialysis, as well as measuring their tissue levels in the ventromedial hypothalamic nucleus (VMH) and lateral hypothalamus (LH) of rats killed by microwave. In the microdialysis perfusate, extracellular levels of ACh and the metabolite choline were increased by 2-DG administration. In the tissue, a dose dependent decrease in the ACh content and a corresponding increase in the choline content were observed in both hypothalamic nuclei 20 min after administration of 2-DG. These data show that cholinergic activity is increased after 2-DG administration. Both the plasma glucose increment and the fluctuation of ACh and choline content were reduced in pentobarbital anesthetized rats. In 6-hydroxydopamine (6-OHDA) pretreated rats, the hypothalamic content of norepinephrine (NE) was reduced to one-third of that in controls, but there was no significant effect on the hyperglycemia or increase in hypothalamic tissue choline levels following 2-DG. Our results suggest the involvement of the hypothalamic cholinergic system in 2-DC-induced hyperglycemia.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
734
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
116-22
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Hypothalamic cholinergic activity associated with 2-deoxyglucose-induced hyperglycemia.
pubmed:affiliation
Department of Neuropsychopharmacology, (Tsumura), Gunma University School of Medicine, Japan.
pubmed:publicationType
Journal Article