Linked Life Data

8892292

Source:http://linkedlifedata.com/resource/pubmed/id/8892292

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-5-16
pubmed:databankReference
pubmed:abstractText
The blood-borne microfilariae of the Brugian nematodes produce multiple isoforms of chitinase, whose expression is coincident with the onset of microfilarial infectivity for mosquitoes. A single cDNA sequence was previously obtained by screening a Brugia malayi microfilarial cDNA library, yet two chitinase isozymes are readily distinguished in this species. In this paper, we present evidence for the existence of multiple transcripts encoding Brugian microfilarial chitinases. Using primers based on the previously-sequenced cDNA clone, we amplified and sequenced two discrete products from B. malayi microfilarial RNA by RT-PCR. While the shorter fragment was nearly identical to the previously sequenced cDNA, the larger fragment contained an extra copy of a serine/threonine-rich repeat. RNAse protection assays were used to demonstrate that both sequences represent true transcripts, and not PCR artifacts. Using primers based on the B.malayi sequence, two novel sequences were generated by RT-PCR from B. pahangi microfilariae. Homologous and cross-species RNAse protection assays verified that multiple transcripts also encode chitinase isozymes in B. pahangi microfilariae.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0166-6851
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
149-58
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Discrete transcripts encode multiple chitinase isoforms in Brugian microfilariae.
pubmed:affiliation
Department of Biology, Tufts University, Medford, MA 02155, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't