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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1997-3-3
|
| pubmed:abstractText |
For studies on processing and tissue distribution of type X collagen, monoclonal antibodies were prepared against human recombinant collagen type X (hrCol X) and tested by ELISA, immunoblotting and immunohistology. Forty-two clones were obtained which were grouped into four different subsets based on their reactivity against native and denatured hrCol X, pepsin-treated hrCol X, and the C-terminal NC-1 domain. Here we present results obtained with four monoclonal antibodies: Clone X 53, a representative of group I, binds with high affinity to both native and pepsin-digested hrCol X but with low affinity to the NC-1 dimer; monoclonal antibodies of group II and III recognized native and denatured hrCol X but not NC-1; antibodies of group II, but not III, reacted to some extent with pepsin treated hrCol X; one antibody (X 34) was obtained that reacted strongly with the isolated NC-1 dimer and native hrCol X but not with the NC-1 monomer or pepsin-digested hrCol X (group IV). Antibodies of all groups stained specifically the hypertrophic zone of fetal human epiphyseal cartilage. Mab X 53 stained the peri- and extracellular matrix of hypertrophic chondrocytes in the lower hypertrophic zone and in the calcified cartilage core in endochondral bone trabecules, while clone X 34 stained intracellularly and the pericellular matrix. All other tissues or cells of the epiphysis were negative. Antibody X 53 reacted also with canine, murine and guinea pig hypertrophic cartilage in tissue sections, but not with bovine or porcine type X collagen. In sections of osteoarthritic cartilage, clusters of hypertrophic chondrocytes in the deep zone were stained, confirming previous observations on enhanced chondrocyte hypertrophy and type X collagen expression in osteoarthritic articular cartilage.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0945-053X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
15
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
231-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8892223-Adult,
pubmed-meshheading:8892223-Animals,
pubmed-meshheading:8892223-Antibodies, Monoclonal,
pubmed-meshheading:8892223-Antibody Specificity,
pubmed-meshheading:8892223-Cartilage, Articular,
pubmed-meshheading:8892223-Cattle,
pubmed-meshheading:8892223-Collagen,
pubmed-meshheading:8892223-Dogs,
pubmed-meshheading:8892223-Fetal Death,
pubmed-meshheading:8892223-Fetus,
pubmed-meshheading:8892223-Gestational Age,
pubmed-meshheading:8892223-Humans,
pubmed-meshheading:8892223-Hypertrophy,
pubmed-meshheading:8892223-Immunoblotting,
pubmed-meshheading:8892223-Immunohistochemistry,
pubmed-meshheading:8892223-Mice,
pubmed-meshheading:8892223-Mice, Inbred BALB C,
pubmed-meshheading:8892223-Osteoarthritis,
pubmed-meshheading:8892223-Protein Denaturation,
pubmed-meshheading:8892223-Recombinant Proteins,
pubmed-meshheading:8892223-Swine
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Immunolocalization of type X collagen in normal fetal and adult osteoarthritic cartilage with monoclonal antibodies.
|
| pubmed:affiliation |
Institute of Experimental Medicine, Friedrich-Alexander-University of Erlangen-Nuremberg, Germany.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|