Linked Life Data

8890731

Source:http://linkedlifedata.com/resource/pubmed/id/8890731

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1996-12-9
pubmed:abstractText
Interleukin 10 was found to prevent cytokine-induced nitric oxide production in murine macrophages. Because, in rat islets, interleukin 1 beta induces beta-cell dysfunction, mainly due to overproduction of nitric oxide, we studied if this effect could be counteracted by interleukin 10. Rat pancreatic islets were cultured for 24 h in the presence or absence of 0.02-20 ng/ml recombinant human interleukin 10. Interleukin 10 dose-dependently inhibited insulin secretion with maximal inhibition (27 +/- 4%, p < 0.05) at 2 ng/ml without impairment of islet cell viability. However, incubation of pancreatic islets with interleukin 10 resulted in a 61.5% decrease of nitric oxide production. Co-incubation of islets with interleukin 10 (2 ng/ml) and recombinant human interleukin 1 beta (0.15 ng/ml) resulted in a more pronounced suppression of basal insulin release than with interleukin 1 beta alone (55 +/- 3.6% vs 44 +/- 3.6% with interleukin 1 beta alone, p < 0.05) but did not reduce interleukin 1 beta-stimulated NO production or reverse the effect of interleukin 1 beta on cell viability. Thus, in pancreatic islets interleukin 10 is not capable of counteracting the interleukin 1 beta induced beta-cell dysfunction, but rather enhances the inhibitory effect of interleukin 1 beta by a different mechanism.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0804-4643
pubmed:author
pubmed:issnType
Print
pubmed:volume
135
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
374-8
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Interleukin 10 inhibits insulin release from and nitric oxide production in rat pancreatic islets.
pubmed:affiliation
Department of Internal Medicine, University Hospital, Zürich, Switzerland.
pubmed:publicationType
Journal Article