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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
8
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pubmed:dateCreated |
1996-12-24
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pubmed:abstractText |
A total of 478 Vibrio parahaemolyticus strains isolated from overseas travellers' diarrhea during the last 7 years of 1989 to 1995 were examined for the production of Kanagawa hemolysin by reversed passive latex agglutination (RPLA) test. Three hundred-seventy (77.4%) out of 478 strains were positive for Kanagawa hemolysin, whereas 108 strains were weakly positive or negative. For those Kanagawa hemolysin-weakly positive or negative strains, the tdh and trh genes associated with the production of TDH (thermostable direct hemolysin) and TRH (TDH-related hemolysin), respectively, were studied by the polymerase chain reaction method. The trh gene was detected in 98 (90.7%) out of 108 strains. In 35 strains belonging to 13 serotypes such as O3: K6, O1:K33, O3:K59, the trh gene alone was detected. On the other hand, both trh and tdh genes were detected in 63 strains of 17 serotypes including O1:K69, O3:K72, O6:K46. Among the strains of 4 serotypes including O1:K56, O1:KUT, O3:KUT and O5:KUT, two types of the trh positive alone and the trh and tdh positive were observed. Of interest, all of the 98 trh-positive strains were positive for the urease hydrolysis, whereas all Kanagawa hemolysin-positive strains were not. Furthermore, the strains of serotype O6:K18 (4 strains) were positive for the fermentation of dulcitol, and the strains of serotype O1:K1 (5 strains) were indole negative. These characteristics of the strains were completely different from those of typical V. parahaemolyticus strains.
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pubmed:language |
jpn
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0387-5911
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
70
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
815-20
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pubmed:dateRevised |
2008-12-16
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pubmed:meshHeading |
pubmed-meshheading:8890549-Diarrhea,
pubmed-meshheading:8890549-Genes, Bacterial,
pubmed-meshheading:8890549-Hemolysin Proteins,
pubmed-meshheading:8890549-Humans,
pubmed-meshheading:8890549-Polymerase Chain Reaction,
pubmed-meshheading:8890549-Serotyping,
pubmed-meshheading:8890549-Travel,
pubmed-meshheading:8890549-Urease,
pubmed-meshheading:8890549-Vibrio parahaemolyticus
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pubmed:year |
1996
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pubmed:articleTitle |
[Detection of the trh gene in Vibrio parahaemolyticus isolated from overseas travellers' diarrhea and their biochemical characteristics].
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pubmed:affiliation |
Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health.
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pubmed:publicationType |
Journal Article,
English Abstract
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