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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1997-1-23
|
| pubmed:abstractText |
The effect of hydrocortisone, sucrose, and epidermal growth factor (EGF), alone or in combination, was investigated on the precocious expression of sucrase activity and sucrase-isomaltase (SI) mRNA in normal and adrenalectomized (ADX) suckling rats. In normal rats, each regulatory factor administered separately from 12 to 13 days of age induced a parallel expression of sucrase activity and SI mRNA. A potentiation of both sucrase activity and SI mRNA was observed after administration of hydrocortisone plus sucrose. Only SI mRNA was potentiated after EGF-sucrose administration or EGF-hydrocortisone administration. However, the potentiation of the activity was obtained after a prolonged treatment period of 4 days. In adrenalectomized rats, sucrose and EGF were still able to induce precocious induction of SI mRNA and sucrase activity, but sucrase activity was lowered by 75% by sucrose and by 30% by EGF. The combined treatments gave results similar to those obtained in normal rats. The results obtained in ADX rats indicate that the effects of sucrose and EGF on the precocious expression of sucrase and the potentiation of SI mRNA are independent of endogenous glucocorticoids. The rate of [3H]thymidine incorporation into mucosal DNA of normal sucklings was similar after single or combined treatments. From this study, we conclude that the precocious induction of intestinal sucrase by various regulatory factors, alone or in combination, is primarily regulated at the level of mRNA and is independent of increases in cellular proliferation and circulating glucocorticoids.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Corticosterone,
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrocortisone,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Sucrase-Isomaltase Complex,
http://linkedlifedata.com/resource/pubmed/chemical/Sucrose
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0277-2116
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
23
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
262-9
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8890076-Adrenalectomy,
pubmed-meshheading:8890076-Animals,
pubmed-meshheading:8890076-Animals, Suckling,
pubmed-meshheading:8890076-Blotting, Northern,
pubmed-meshheading:8890076-Cell Division,
pubmed-meshheading:8890076-Corticosterone,
pubmed-meshheading:8890076-Diet,
pubmed-meshheading:8890076-Epidermal Growth Factor,
pubmed-meshheading:8890076-Epithelial Cells,
pubmed-meshheading:8890076-Female,
pubmed-meshheading:8890076-Gene Expression,
pubmed-meshheading:8890076-Hydrocortisone,
pubmed-meshheading:8890076-Intestines,
pubmed-meshheading:8890076-Male,
pubmed-meshheading:8890076-RNA, Messenger,
pubmed-meshheading:8890076-Rats,
pubmed-meshheading:8890076-Sucrase-Isomaltase Complex,
pubmed-meshheading:8890076-Sucrose
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Sucrase-isomaltase gene expression in suckling rat intestine: hormonal, dietary, and growth factor control.
|
| pubmed:affiliation |
Institut de Recherche Contre les Cancers de l'Appareil Digestif (IRCAD), Hôpitaux Universitaires, Strasbourg, France.
|
| pubmed:publicationType |
Journal Article
|