Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-2-6
pubmed:abstractText
1. The aim of the present study was to investigate whether antimigraine ergot compounds may act at endothelial 5-hydroxytryptamine (5-HT) receptors which trigger the release of endothelium-derived relaxing factor (EDRF). Changes in tone of porcine isolated pulmonary arteries were measured isometrically. The integrity of the endothelium was assessed by the bradykinin-induced relaxation of prostaglandin F2 alpha (PGF2 alpha, 3 microM)-precontracted vessels. 2. The ergot derivatives ergotamine, dihydroergotamine (DHE) and dihydroergocristine, as well as 5-HT and (+/-)-alpha-methyl-5-HT, elicited a reversible endothelium-dependent relaxation of PGF2 alpha-precontracted arterial ring segments. The relaxation to both ergotamine and 5-HT was associated with an increase in cyclic GMP. After pretreatment of the vessels with NG-nitro-L-arginine methyl ester (200 microM), or removal of endothelium by mechanical rubbing, the relaxant responses were abolished. 3. The mean pEC50 values for relaxant responses followed the order: (+/-)-alpha-methyl-5-HT (8.80) > 5-HT (8.75) > ergotamine (8.17) > DHE (7.70) > 5-carboxamidotryptamine (7.62) > dihydroergocristine (7.17). 4. The relaxant effects of both ergotamine and dihydroergotamine were resistant to block by indomethacin (3 microM), prazosin (1 microM) and ketanserin (1 microM). However, the ergotamine-induced relaxation was highly susceptible to block by pizotifen (pA2 = 8.23), norclozapine (pA2 = 8.20), methiothepin (-log IC50 = 7.26), rauwolscine (pA2 = 7.24) and mesulergine (pA2 = 6.64). Each antagonist inhibited the relaxant responses to (+/-)-alpha-methyl-5-HT in the same manner with similar potency as that determined against ergotamine. 5. Recently, mRNA transcripts for 5-HT1D beta and 5-HT2B receptors have been demonstrated in porcine pulmonary arteries. The rank order of potencies of agonists and antagonists in the present study suggests that the relaxant responses to 5-HT and ergot derivatives are mediated through activation of endothelial 5-HT receptors which are similar to the 5-HT2B receptor subtypes.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-13996119, http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-1505525, http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-1786517, http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-2127899, http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-2137176, http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-2812041, http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-2904153, http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-6096723, http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-6505006, http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-7538702, http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-7582481, http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-7656980, http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-7704102, http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-7792930, http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-7824037, http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-7881740, http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-7926008, http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-7938165, http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-7972337, http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-8107508, http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-8143856, http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-8387927, http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-8391650, http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-8395342, http://linkedlifedata.com/resource/pubmed/commentcorrection/8886417-8437119
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0007-1188
pubmed:author
pubmed:issnType
Print
pubmed:volume
119
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
330-4
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Endothelial 5-HT receptors mediate relaxation of porcine pulmonary arteries in response to ergotamine and dihydroergotamine.
pubmed:affiliation
University Jena, Medical Faculty, Center for Vascular Biology and Medicine, Erfurt, Germany.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't