Linked Life Data

8886002

Source:http://linkedlifedata.com/resource/pubmed/id/8886002

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1996-12-9
pubmed:abstractText
In some pedigrees of familial Alzheimer's disease (FAD), three mutations of beta amyloid precursor protein (APP) have been found at the Val717 residue (to Ile, Phe, or Cly) and these mutations increase the secretion of A beta 42(43). To study the specificity of the effects of these mutations on APP processing, we transiently expressed APP genes with mutations of Val717 to Lys, Ser, Glu, or Cys in COS cells. The three familial AD-linked mutations increased the levels or ratios of A beta 42(43), whereas the secretion of A beta 40 was decreased. Other mutations irrelevant to FAD except Val717 to Lys had little effect on the ratio of A beta 42(43). Substitution to Lys decreased the secretion of A beta 42(43); substitution to Glu or Gly decreased the amount of intracellular C-terminal fragment produced by alpha-secretase, whereas it was increased by mutations to Phe, Cys, or Lys. However, the levels of secretion of soluble APP were constant, but a substitution to Glu reduced it. These results suggest a specific role of the Val717 residue in APP processing and, especially, in gamma-cleavage.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
227
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
730-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Familial Alzheimer's disease-linked mutations at Val717 of amyloid precursor protein are specific for the increased secretion of A beta 42(43).
pubmed:affiliation
Laboratory of Neurochemistry, National Institute for Physiological Sciences, Aichi, Japan. keimaru@nips.ac.;p
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't