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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1996-12-4
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pubmed:abstractText |
Cytokine regulation of B cell development was analyzed using interleukin-2 (IL-2)-induced transcription of the J chain gene as a model system. A nuclear target of the IL-2 signal was identified as the Pax5 transcription factor, BSAP, which recognizes a negative regulatory motif in the J chain promoter. Functional assays showed that BSAP mediates the silencing of the J chain gene during the early stages of B cell development, but repression is relieved during the antigen-driven stages in a concentration-dependent manner by an IL-2-induced down-regulation of BSAP RNA expression. At the low levels present in J chain-expressing plasma cells, BSAP repression could be overridden by positive-acting factors binding to down-stream J chain promoter elements. Overexpression of BSAP in these cells reversed the positive regulation and inhibited J chain gene transcription. Thus, IL-2 regulation of BSAP concentration may provide a mechanism for controlling both repressor and activator functions of BSAP during a B cell immune response.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1074-7613
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
377-86
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8885870-Animals,
pubmed-meshheading:8885870-B-Cell-Specific Activator Protein,
pubmed-meshheading:8885870-B-Lymphocytes,
pubmed-meshheading:8885870-Cell Differentiation,
pubmed-meshheading:8885870-Cells, Cultured,
pubmed-meshheading:8885870-DNA-Binding Proteins,
pubmed-meshheading:8885870-Gene Expression Regulation,
pubmed-meshheading:8885870-Genes, Immunoglobulin,
pubmed-meshheading:8885870-Immunoglobulin J-Chains,
pubmed-meshheading:8885870-Interleukin-2,
pubmed-meshheading:8885870-Mice,
pubmed-meshheading:8885870-Nuclear Proteins,
pubmed-meshheading:8885870-Promoter Regions, Genetic,
pubmed-meshheading:8885870-Repressor Proteins,
pubmed-meshheading:8885870-Sequence Deletion,
pubmed-meshheading:8885870-Transcription Factors
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pubmed:year |
1996
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pubmed:articleTitle |
An interleukin-2 signal relieves BSAP (Pax5)-mediated repression of the immunoglobulin J chain gene.
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pubmed:affiliation |
Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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