Linked Life Data

8885089

Source:http://linkedlifedata.com/resource/pubmed/id/8885089

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1997-2-10
pubmed:abstractText
Bacterial lipopolysaccharide (LPS) can induce hyporesponsiveness to its own toxic effects, as reflected by the diminished production of tumor necrosis factor (TNF) and other inflammatory mediators by animals (and by purified macrophages) upon re-challenge with LPS. We have examined the regulation of TNF and inducible nitric oxide synthase (iNOS) production in the murine macrophage cell line RAW 264.7. These cells accumulated TNF mRNA and secreted TNF protein in a dose-dependent fashion after exposure to either LPS or recombinant murine interferon-gamma (riFN-gamma). Pre-exposure of RAW 264.7 cells to relatively high concentrations of LPS (> 10 ng/mL) resulted in diminished production of TNF in response to subsequent challenge with either LPS or riFN-gamma. In contrast, production of iNOS mRNA was either unaffected or augmented by pre-exposure of these cells to LPS. Our findings indicate that the hyporesponsive state of RAW 264.7 macrophages preincubated with LPS is not specific for LPS and that the production of TNF and nitric oxide (NO) by macrophages are differentially regulated.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1073-2322
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
218-22
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Acquired hyporesponsiveness to bacterial lipopolysaccharide and interferon-gamma in RAW 264.7 macrophages.
pubmed:affiliation
Department of Pediatrics, University of Tennessee, Memphis, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't