Linked Life Data

8879236

Source:http://linkedlifedata.com/resource/pubmed/id/8879236

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1996-12-10
pubmed:databankReference
pubmed:abstractText
In photoautotrophic organisms it is well documented that the expression of nuclear genes encoding plastid proteins can be regulated at various levels. We present here the analysis of a non-photosynthetic strain (CC1051) of the green unicellular alga Chlamydomonas reinhardtii; this strain carries a mutation in the newly identified Cen gene involved in the co-regulated expression of several different nuclear genes encoding plastid proteins. We performed a differential screening strategy to isolate cDNAs corresponding to genes that are differentially expressed in mutant and wild-type strains. Extensive hybridization experiments revealed that the 15 cDNA clones isolated represent five different mRNAs that fail to accumulate in the non-photosynthetic mutant. Comparative analysis of DNA sequencing data showed that they all code for plastid proteins. In particular, we identified genes for the chlorophyll a/b binding protein of the light-harvesting complex II (LHCII), for subunits II and III of photosystem I (PsaD, PsaF), for pentose-5-phosphate 3-epimerase (PPE), an enzyme of the Calvin cycle, and for an unidentified 7 kDa protein with a suggested lumenal location. With the exception of the gene for LHCII, all proteins are encoded by single-copy genes. Evidence from run-on transcription experiments is presented showing that expression of the above mentioned plastid proteins is affected at the post-transcriptional level in the mutant strain CC1051 with a defect in the Cen gene. Our results suggest that the product of the Cen gene is involved in stabilization and/or processing of transcripts from nuclear genes encoding chloroplast proteins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0026-8925
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
252
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
362-70
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8879236-Amino Acid Sequence, pubmed-meshheading:8879236-Animals, pubmed-meshheading:8879236-Base Sequence, pubmed-meshheading:8879236-Chlamydomonas reinhardtii, pubmed-meshheading:8879236-Chloroplasts, pubmed-meshheading:8879236-Cloning, Molecular, pubmed-meshheading:8879236-DNA, Complementary, pubmed-meshheading:8879236-Fungal Proteins, pubmed-meshheading:8879236-Gene Expression Regulation, Fungal, pubmed-meshheading:8879236-Molecular Sequence Data, pubmed-meshheading:8879236-Mutation, pubmed-meshheading:8879236-Nuclear Proteins, pubmed-meshheading:8879236-Peptides, pubmed-meshheading:8879236-Photosynthesis, pubmed-meshheading:8879236-Plastids, pubmed-meshheading:8879236-RNA, Messenger, pubmed-meshheading:8879236-Sequence Analysis, DNA, pubmed-meshheading:8879236-Transcription, Genetic
pubmed:year
1996
pubmed:articleTitle
Altered expression of nuclear genes encoding chloroplast polypeptides in non-photosynthetic mutants of Chlamydomonas reinhardtii: evidence for post-transcriptional regulation.
pubmed:affiliation
Lehrstuhl für Allgemeine Botanik, Ruhr-Universität Bochum, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't