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rdf:type |
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lifeskim:mentions |
umls-concept:C0011847,
umls-concept:C0017296,
umls-concept:C0024264,
umls-concept:C0026336,
umls-concept:C0079429,
umls-concept:C0085243,
umls-concept:C0205734,
umls-concept:C0348011,
umls-concept:C1423842,
umls-concept:C1705822,
umls-concept:C1708528
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pubmed:issue |
8
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pubmed:dateCreated |
1996-11-27
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pubmed:abstractText |
Four pancreatic islet-specific CD4+ helper T (Th) 1 (Th1) clones and two Th1 clones transduced with an SRalpha promoter-linked murine IL-10 (mIL-10) cDNA of 2.0-6.0 x 10(6) cells were adoptively transferred to nonobese diabetic (NOD) mice at age 8 d. Cyclophosphamide (CY) was administered at age 37 d (plus CY), and the incidence of diabetes and the histological grade of insulitis were examined at age 47 d. After the adoptive transfer of IL-10-transduced Th1 cells, polymerase chain reaction (PCR) and reverse-transcription (RT)-PCR detected the neo gene and the retrovirus vector-mediated IL-10 mRNA in situ in recipient islets, respectively. RT-PCR detected the decrease of IFN-gamma mRNA relative to IL-10 mRNA in IL-10-transduced Th1 clones in vitro and also in recipient islets. All four wild type Th1 clones plus CY induced the insulitis grade of 2.75 and diabetes in 66% of recipient NOD mice. IL-10-transduced two Th1 clones plus CY induced periinsulitis with the grade of 1.43 and diabetes in 8.0%. The 1:1 mixture of wild type Th1 cells and IL-10-transduced Th1 cells plus CY induced periinsulitis with the grade of 1.85 and diabetes in 20%. The suppression of diabetes through decreasing IFN-gamma mRNA by the tissue-specific delivery of IL-10 to pancreatic islets with IL-10-transduced Th1 cells affords us the starting basis to develop the gene therapy for autoimmune diabetes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-1422257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-1628770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-1899431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-1902501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-1909135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-2188676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-2194165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-2205920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-2307481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-2405400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-2449974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-2510155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-2523712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-2533502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-2661286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-2827008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-2965652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-3046964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-3121415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-3134263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-3275717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-3309126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-3413107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-3472348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-3525284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-3901813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-6530055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-7696210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-7761837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-8064245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-8073945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-8100367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-8104408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-8132775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-8145050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-8181185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-8215637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-8315397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-8432410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-8476563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8878437-8551245
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
98
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1851-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8878437-Adoptive Transfer,
pubmed-meshheading:8878437-Animals,
pubmed-meshheading:8878437-Cyclophosphamide,
pubmed-meshheading:8878437-Diabetes Mellitus, Type 1,
pubmed-meshheading:8878437-Gene Therapy,
pubmed-meshheading:8878437-Gene Transfer Techniques,
pubmed-meshheading:8878437-Interferon-gamma,
pubmed-meshheading:8878437-Interleukin-10,
pubmed-meshheading:8878437-Islets of Langerhans,
pubmed-meshheading:8878437-Mice,
pubmed-meshheading:8878437-Mice, Inbred NOD,
pubmed-meshheading:8878437-Polymerase Chain Reaction,
pubmed-meshheading:8878437-RNA, Messenger,
pubmed-meshheading:8878437-Th1 Cells
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pubmed:year |
1996
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pubmed:articleTitle |
Prevention of adoptively transferred diabetes in nonobese diabetic mice with IL-10-transduced islet-specific Th1 lymphocytes. A gene therapy model for autoimmune diabetes.
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pubmed:affiliation |
Otsuka Department of Clinical and Molecular Nutrition, School of Medicine, The University of Tokushima, Japan.
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