Linked Life Data

8878208

Source:http://linkedlifedata.com/resource/pubmed/id/8878208

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1997-1-23
pubmed:abstractText
As cell cultures of pericytes derived from bovine retina are in regular use by a number of investigators, we decided to confirm the identity of these cells, to show them different from other cells that might contaminate cultures derived from retinal tissue, to characterize the properties they share with pericytes in situ and to establish a practical and effective means for confirmation of the cell type of each batch of cultured cells used in scientific studies. Cultured pericytes differed in immunocytochemical reactions and phagocytic properties from vascular smooth muscle, vascular endothelial cells, astroglia, conjunctival fibroblasts or retinal pigment epithelium. Like vascular smooth muscle, pericytes in situ and those in culture have the contractile proteins actin and myosin, which implies a potential role in blood flow control. In routine studies of cultured pericytes, they can be identified by their unique reaction in situ and in vitro with the antiganglioside monoclonal antibody 3G5. The presence of any contaminating endothelial cells can be recognized by their ability to take up acetylated low-density lipoprotein.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0030-3755
pubmed:author
pubmed:issnType
Print
pubmed:volume
210
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
263-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Glaucoma, capillaries and pericytes. 2. Identification and characterization of retinal pericytes in culture.
pubmed:affiliation
Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami School of Medicine, Fla., USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't