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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
1996-12-4
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pubmed:abstractText |
Brn-4 is a member of the POU transcription factor family and is expressed in the central nervous system. In this study, we addressed whether Brn-4 regulates expression of the D1A dopamine receptor gene. We found a functional Brn-4 responsive element in the intron of this gene by means of cotransfection chloramphenical acetyltransferase assays. This region contains two consensus sequences for binding of POU factors. Gel mobility-shift assays using glutathione S-transferase-Brn-4 fusion protein indicated that Brn-4 binds to these sequences. Both these sites are essential for transactivation by Brn-4 because deletion of either significantly reduced this enhancer activity. In situ hybridization revealed colocalization of Brn-4 and D1A mRNAs at the level of a single neuron in the rat striatum where this dopamine receptor is most abundantly expressed. Gel mobility-supershift assay using rat striatal nuclear extract and Brn-4 antibody confirmed the presence of Brn-4 in this brain region and its ability to bind to its consensus sequences in the D1A gene. These data suggest a functional role for Brn-4 in the expression of the D1A dopamine receptor gene both in vitro and in vivo.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1302000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1348858,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1361172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1402930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1483387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1509262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1509263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1511979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1557411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1565620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1584227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1610826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1628619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1680563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1690859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1695398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1705013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1825729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1915274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1967821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1967980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1972777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1975954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1976514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-1977085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-2044958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-2082192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-2350782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-2379827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-2561978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-2665904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-2739723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-3215510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-7637804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-7690239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-7809612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-7903601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-7904822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-7954836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-8001143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-8106170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-8144606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-8226883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-8248179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-8274283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-8352967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876240-8503439
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
93
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11933-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8876240-Animals,
pubmed-meshheading:8876240-Base Sequence,
pubmed-meshheading:8876240-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:8876240-Cytomegalovirus,
pubmed-meshheading:8876240-DNA Primers,
pubmed-meshheading:8876240-Gene Expression Regulation,
pubmed-meshheading:8876240-Genes, Reporter,
pubmed-meshheading:8876240-Genetic Vectors,
pubmed-meshheading:8876240-Glutathione Transferase,
pubmed-meshheading:8876240-Oligonucleotide Probes,
pubmed-meshheading:8876240-Polymerase Chain Reaction,
pubmed-meshheading:8876240-Rats,
pubmed-meshheading:8876240-Receptors, Dopamine D1,
pubmed-meshheading:8876240-Recombinant Fusion Proteins,
pubmed-meshheading:8876240-Transcription, Genetic,
pubmed-meshheading:8876240-Transcription Factors,
pubmed-meshheading:8876240-Transfection
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pubmed:year |
1996
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