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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
1996-12-4
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pubmed:abstractText |
A recombinant rabies virus (RV) mutant deficient for the surface spike glycoprotein (G) gene was used to study the incorporation of envelope proteins from HIV-1 expressed from transfected plasmids. A hybrid HIV-1 protein in which the cytoplasmic domain was replaced with that of RV G was incorporated into the virus envelope and rescued the infectivity of the RV mutant. The RV(HIV-1) pseudotype viruses could infect only CD4+ cells, and their infectivity was neutralized specifically by anti-HIV-1 sera. In contrast to the chimeric protein, wild-type HIV-1 envelope protein or mutants with truncated cytoplasmic domains failed to produce pseudotyped particles. This indicates the presence of a specific signal in the RV G cytoplasmic domain, allowing correct incorporation of a spike protein into the envelope of rhabdovirus particles. The possibility of directing the cell tropism of RV by replacement of the RV G with proteins of defined receptor specificity should prove useful for future development of targetable gene delivery vectors.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-1310767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-1331528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-1546457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-1652977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-1812946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-2431105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-2547986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-2676191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-2684654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-2849111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-2981635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-3019669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-3094962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-3095828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-3413127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-6255080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-7853476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-7925265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-7937806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-7954815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-8093220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-8289375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-8396259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-8601317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-8601771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-8642658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8876141-8692989
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
93
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11366-70
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8876141-Amino Acid Sequence,
pubmed-meshheading:8876141-Antigens, CD,
pubmed-meshheading:8876141-Antigens, CD4,
pubmed-meshheading:8876141-Gene Deletion,
pubmed-meshheading:8876141-Gene Products, env,
pubmed-meshheading:8876141-Genetic Complementation Test,
pubmed-meshheading:8876141-Genetic Vectors,
pubmed-meshheading:8876141-HIV-1,
pubmed-meshheading:8876141-HeLa Cells,
pubmed-meshheading:8876141-Humans,
pubmed-meshheading:8876141-Membrane Glycoproteins,
pubmed-meshheading:8876141-Molecular Sequence Data,
pubmed-meshheading:8876141-Rabies virus,
pubmed-meshheading:8876141-Recombinant Proteins,
pubmed-meshheading:8876141-Restriction Mapping,
pubmed-meshheading:8876141-Vaccinia virus,
pubmed-meshheading:8876141-Viral Matrix Proteins
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pubmed:year |
1996
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pubmed:articleTitle |
Specific infection of CD4+ target cells by recombinant rabies virus pseudotypes carrying the HIV-1 envelope spike protein.
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pubmed:affiliation |
Department of Clinical Virology, Federal Research Centre for Virus Diseases of Animals, Tübingen, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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