| pubmed-article:8875950 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8875950 | lifeskim:mentions | umls-concept:C1522102 | lld:lifeskim |
| pubmed-article:8875950 | lifeskim:mentions | umls-concept:C1513095 | lld:lifeskim |
| pubmed-article:8875950 | lifeskim:mentions | umls-concept:C0035696 | lld:lifeskim |
| pubmed-article:8875950 | lifeskim:mentions | umls-concept:C0002302 | lld:lifeskim |
| pubmed-article:8875950 | lifeskim:mentions | umls-concept:C0041484 | lld:lifeskim |
| pubmed-article:8875950 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:8875950 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:8875950 | pubmed:dateCreated | 1996-11-27 | lld:pubmed |
| pubmed-article:8875950 | pubmed:abstractText | Cloudman S-91 mouse melanoma cells respond to alpha-melanocyte-stimulating hormone) by demonstrating a marked increase in tyrosinase activity (O-diphenol-O2 oxidoreductase, EC 1.14.18.1). This increase is the result of increased levels of tyrosinase mRNA with a subsequent increase in tyrosinase abundance. Our studies were carried out to determine the effect of melanocyte-stimulating hormone on tyrosinase gene transcription and to measure the kinetics of the hormone-induced increase in tyrosinase mRNA. When melanoma cells were exposed continuously to melanocyte-stimulating hormone for 6 d, a large but transient increase in both tyrosinase mRNA abundance and enzyme activity were observed. The maximum increase in tyrosinase mRNA occurred 60 h after melanocyte-stimulating hormone stimulation and was followed by a decline in message levels even though cells were continuously exposed to hormone. Results of nuclear run-off transcription assays showed that melanocyte-stimulating hormone caused a slow increase in the rate of transcription of the tyrosinase gene with a maximal 6-fold stimulation occurring at 48 h. In cells treated with the ribonucleic acid synthesis inhibitor, 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole, tyrosinase mRNA levels decayed with a half-life of 4-5 h. This decay rate was unaffected by treatment of cells with melanocyte-stimulating hormone, indicating that the hormone does not act to stabilize tyrosinase ribonucleic acid. Inhibition of protein synthesis by treatment with cycloheximide had no effect on the melanocyte-stimulating hormone-induced increase in tyrosinase messenger ribonucleic acid levels suggesting that ongoing protein synthesis is not required for, at least, the initial stimulation of tyrosinase gene transcription by melanocyte-stimulating hormone. | lld:pubmed |
| pubmed-article:8875950 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8875950 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8875950 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8875950 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8875950 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8875950 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8875950 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8875950 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8875950 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8875950 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8875950 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:8875950 | pubmed:issn | 0022-202X | lld:pubmed |
| pubmed-article:8875950 | pubmed:author | pubmed-author:FullerB BBB | lld:pubmed |
| pubmed-article:8875950 | pubmed:author | pubmed-author:RungtaDD | lld:pubmed |
| pubmed-article:8875950 | pubmed:author | pubmed-author:CornT DTD | lld:pubmed |
| pubmed-article:8875950 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8875950 | pubmed:volume | 107 | lld:pubmed |
| pubmed-article:8875950 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8875950 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8875950 | pubmed:pagination | 689-93 | lld:pubmed |
| pubmed-article:8875950 | pubmed:dateRevised | 2005-11-17 | lld:pubmed |
| pubmed-article:8875950 | pubmed:meshHeading | pubmed-meshheading:8875950-... | lld:pubmed |
| pubmed-article:8875950 | pubmed:meshHeading | pubmed-meshheading:8875950-... | lld:pubmed |
| pubmed-article:8875950 | pubmed:meshHeading | pubmed-meshheading:8875950-... | lld:pubmed |
| pubmed-article:8875950 | pubmed:meshHeading | pubmed-meshheading:8875950-... | lld:pubmed |
| pubmed-article:8875950 | pubmed:meshHeading | pubmed-meshheading:8875950-... | lld:pubmed |
| pubmed-article:8875950 | pubmed:meshHeading | pubmed-meshheading:8875950-... | lld:pubmed |
| pubmed-article:8875950 | pubmed:meshHeading | pubmed-meshheading:8875950-... | lld:pubmed |
| pubmed-article:8875950 | pubmed:meshHeading | pubmed-meshheading:8875950-... | lld:pubmed |
| pubmed-article:8875950 | pubmed:meshHeading | pubmed-meshheading:8875950-... | lld:pubmed |
| pubmed-article:8875950 | pubmed:meshHeading | pubmed-meshheading:8875950-... | lld:pubmed |
| pubmed-article:8875950 | pubmed:meshHeading | pubmed-meshheading:8875950-... | lld:pubmed |
| pubmed-article:8875950 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8875950 | pubmed:articleTitle | Regulation of tyrosinase mRNA in mouse melanoma cells by alpha-melanocyte-stimulating hormone. | lld:pubmed |
| pubmed-article:8875950 | pubmed:affiliation | Department of Biochemistry and Molecular Biology, University of Oklahoma, Health Science Center, Oklahoma City, USA. | lld:pubmed |
| pubmed-article:8875950 | pubmed:publicationType | Journal Article | lld:pubmed |
| entrez-gene:22173 | entrezgene:pubmed | pubmed-article:8875950 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8875950 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8875950 | lld:pubmed |
