Linked Life Data

8875811

Source:http://linkedlifedata.com/resource/pubmed/id/8875811

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1996-12-9
pubmed:abstractText
Unaveraged tomograms of embedded and sectioned insect flight muscle show not only structural variation within the individual unit cells but also defects in the filament lattice such as lack of straightness of the filaments and wrinkling of the sections. Adaptation of conventional 2D crystal image processing methods usually average out these structural variations and lattice defects thereby reducing the resolution and information content of the 3D reconstructions. We present here a computational method to correct the reconstructed 3D object for these distortions to make it suitable for further analysis. The correction is an extension of conventional 2D cross-correlation techniques applied to a 3D image. These procedures determine the unit cell positions of the distorted crystal and use a spline-fitting approach to calculate a mapping function from the observed positions that is subsequently used to reinterpolate the image onto a regular grid. The resulting reinterpolated image is corrected for both filament straightening as well as section wrinkling.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0304-3991
pubmed:author
pubmed:issnType
Print
pubmed:volume
63
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
125-32
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Three-dimensional distortion correction applied to tomographic reconstructions of sectioned crystals.
pubmed:affiliation
Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't