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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1996-12-3
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pubmed:databankReference |
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pubmed:abstractText |
The complete nucleotide sequence of the gene encoding the major outer capsid protein VP2 from the Alberta isolate of epizootic hemorrhagic disease virus serotype 2 (EHDV-2) was determined. Complementary DNA (cDNA) corresponding to segment 2 was 3002 nucleotides in length with a single open reading frame that encoded a VP2 of 982 amino acids. Although the VP2 from EHDV-2 was only 34% homologous to the cognate protein from EHDV-1, their predicted hydropathic profiles were similar, suggesting that conservation of structure is important biologically to these capsid proteins. Sequence analysis of six North American EHDV-2 field isolates showed a high degree of comparative genetic identity (> 97%). Phylogenetic profiles constructed suggest that regionalization of the viruses within the North American continent has contributed to the genetic diversity.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0042-6822
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
224
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
555-60
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8874517-Amino Acid Sequence,
pubmed-meshheading:8874517-Animals,
pubmed-meshheading:8874517-Capsid,
pubmed-meshheading:8874517-Capsid Proteins,
pubmed-meshheading:8874517-Genetic Variation,
pubmed-meshheading:8874517-Hemorrhagic Disease Virus, Epizootic,
pubmed-meshheading:8874517-Molecular Sequence Data,
pubmed-meshheading:8874517-Phylogeny,
pubmed-meshheading:8874517-RNA, Viral,
pubmed-meshheading:8874517-Sequence Analysis, RNA,
pubmed-meshheading:8874517-Sequence Homology, Amino Acid,
pubmed-meshheading:8874517-Serotyping
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pubmed:year |
1996
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pubmed:articleTitle |
Molecular characterization of the segment 2 gene of epizootic hemorrhagic disease virus serotype 2: gene sequence and genetic diversity.
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pubmed:affiliation |
Department of Molecular Biology, University of Wyoming, Laramie 82071, USA.
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pubmed:publicationType |
Journal Article
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