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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1997-2-3
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pubmed:abstractText |
Three different procedures were used to isolate lipopolysaccharides from the Salmonella enteritidis strain 477: phenol-water extraction with ethanol precipitation (LPS 1), phenol-water extraction with methanol precipitation (LPS 2) and FPLC purification (LPS 1/1). Production of interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) was observed in the supernatants of adherent spleen cells of BALB/c mice after the stimulation and cultivation of the cells. The quantity of IL-1 beta and IL-6 depended on the method of LPS isolation. The highest level of IL-1 beta was recorded at LPS 2, and of IL-6 at the stimulation of cells by means of LPS 1.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0892-3973
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
433-41
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pubmed:dateRevised |
2009-5-14
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pubmed:meshHeading |
pubmed-meshheading:8872494-Animals,
pubmed-meshheading:8872494-Cells, Cultured,
pubmed-meshheading:8872494-Female,
pubmed-meshheading:8872494-Interleukin-1,
pubmed-meshheading:8872494-Interleukin-6,
pubmed-meshheading:8872494-Lipopolysaccharides,
pubmed-meshheading:8872494-Mice,
pubmed-meshheading:8872494-Mice, Inbred BALB C,
pubmed-meshheading:8872494-Salmonella enteritidis,
pubmed-meshheading:8872494-Spleen
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pubmed:year |
1996
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pubmed:articleTitle |
Production of IL-1 beta and IL-6 by adherent spleen cells after the stimulation with lipopolysaccharides from Salmonella enteritidis strain.
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pubmed:affiliation |
University of Veterinary Medicine, Department of Microbiology and Immunology, Kosice, Slovak Republic.
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pubmed:publicationType |
Journal Article
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